Fao Cell Line from rat

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89042701, rat liver, Hepatocyte

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: rat liver
• Verpackung: tube of 5 μg 89042701-DNA-5UG; pkg of vial of cells 89042701-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Hepatocyte
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat hepatoma

Beschreibung der Zelllinie

A differentiated cell line derived from H4-11-E-C3 (Sigma catalogue no. 85061112). A versatile tool for studying regulation of mono-oxygenase activities and analysing their role in the activation of xenobiotics e.g. carcinogens, drugs and pesticides.

Anwendung

Differentiation and enzymatic studies

Nährmedium

Coon′s modified Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) or kaign′s modified Ham′s F12 + 45mg/l asorbic acid +18mg/1 inositol + 10% FBS +2mM Glutamine

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂ 37°C.

Sonstige Hinweise

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