Huh-7D 12 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA. 01042712, human liver, Epithelial

• Synonym(e): Huh7 Cells, JTC-39 Cells, Japanese Tissue Culture-39 Cells
• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human liver
• Verpackung: tube of 5 μg 01042712-DNA-5UG; vial of cells 01042712-1VL
• Wachstumsmodus: Adherent
• Karyotyp: That of original Huh-7
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Lagertemp.: −196°C

Beschreibung

Allgemeine Beschreibung

Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised. Huh-7D is a derivative of Huh-7 cells, which favor hepatitis C virus replication. Huh-7 cells are superior hepatic cell models compared to HepG2 cells for apolipoprotein based metabolic studies.

Ursprung der Zelllinie

Human hepatocellular carcinoma

Beschreibung der Zelllinie

Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised.

Anwendung

Study of intracellular traffic of delta virus antigens and RNA, viral RNA replication and early virus assembly events, and interaction between HDV and HBV.

Verpackung

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 10
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 7
TPOX: 8,11
vWA: 16,18

Nährmedium

DMEM (D6546) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37 C. Media change twice a week

Sonstige Hinweise

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