SK-OV-3 Cell Line human

91091004, human ovary, Epithelial

• Synonym(e): SKOV-3 Cells, SKOV3 Cells
• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: SK-OV-3 Cell Line human, 91091004
• Biologische Quelle: human ovary
• Beschreibung: Human Caucasian ovary adenocarcinoma
• Wachstumsmodus: Adherent
• Karyotyp: Hypodiploid to hypotetraploid
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian ovary adenocarcinoma

Beschreibung der Zelllinie

Derived from the ascitic fluid from a 64 year old caucasian female with an ovarian tumour. Forms moderately well-differentiated adenocarcinoma consistent with ovarian primary cells.

Anwendung

SK-OV-3 cell line from humans has been used to study chimeric adenoviruses of Ad5 (species C) displaying proteins of the naturally enteric Ad40 (species F) as vectors for gene delivery.

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 8,11
D16S539: 12
D5S818: 11
D7S820: 13,14
THO1: 9,9.3
TPOX: 8,11
vWA: 17,18

Nährmedium

McCoy′s 5a + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-6x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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