59M

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Synonym(e): OAW 59M Cells, OAW59M Cells
• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human ovary
• Verpackung: tube of 5 μg 89081802-DNA-5UG; pkg of vial of cells 89081802-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human ovarian tumour epithelial

Beschreibung der Zelllinie

Derived from a patient with carcinoma of the ovary and is of ascitic fluid origin.

Anwendung

Tumourigenicity studies

Nährmedium

DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 20 IU/l Bovine Insulin + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Dr A Wilson, Oncology Research Laboratory, Derby City Hospital