ARH 77

88121201, human blood, Lymphoblast

• UNSPSC-Code: 41106514

Eigenschaften

• product name: ARH 77, 88121201
• Biologische Quelle: human blood
• Wachstumsmodus: Suspension
• Karyotyp: Hypodiploid
• Morphologie: Lymphoblast
• Produkte: Immunoglobulin G (IgG1) (kappa)
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human plasma cell leukaemia

Beschreibung der Zelllinie

Derived from a 33 year old female with plasma cell leukaemia. The cells synthesise IgG1 (kappa-light chain) and are EBNA positive.Although the cells are reported to originate from a patient with a plasma cell leukaemia , it has now been shown that this is in fact an EBV transformed B lymphoblastoid cell line .

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 6,10
D13S317: 11,13
D16S539: 9,13
D5S818: 10,13
D7S820: 7,12
THO1: 8,9.3
TPOX: 8
vWA: 17

Nährmedium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Maintain cultures between 1-4x100,000 cells/ml; 5% CO₂; 37°C. Maximum cell density 1-2x1,000,000 cells/ml.

Sonstige Hinweise

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