Jurkat E6.1 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88042803, human blood (leukemic T-cell lymphoblast), Not specified

• Synonym(e): Jurkat Cells, Jurkat Clone E6-1 Cells
• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human blood (leukemic T-cell lymphoblast)
• Verpackung: tube of 5 μg 88042803-DNA-5UG; pkg of vial of cells 88042803-1VL
• Wachstumsmodus: Suspension
• Karyotyp: Pseudodiploid, modal No 46
• Morphologie: Not specified
• Produkte: Interleukin 2 (IL-2), human alpha interferon
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human leukaemic T cell lymphoblast

Beschreibung der Zelllinie

Derived from Jurkat FHCRC. An IL-2 producing cell line, derived by incubating the cells at 41°C for 48 hours followed by a limiting dilution cloning over macrophages.

Anwendung

IL-2 assay and cloning studies

Jurkat E6.1 cell line has been used to:

• study cell-independent/vector-free delivery of bovine serum albumin-fluorescein conjugate
• determine the cytotoxicity of the cytolethal distending toxin (CDT) holotoxin and its components
• study the expression of bisphenol A exposed estrogen receptor-β (ERβ) and estrogen-related-receptor-α (ERRα) in vitro

DNA-Profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18

Nährmedium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. On resuscitation single cells can be observed, during culture most cells will grow as aggregates.

Sonstige Hinweise

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.