MRC-5 (PD 30) Cell Line human

5090501, human lung, Fibroblast-like

• Synonym(e): MRC-5 (PD30) Cells
• UNSPSC-Code: 41106514

Eigenschaften

• product name: MRC-5 (PD 30) Cell Line human, 05090501
• Biologische Quelle: human lung
• Beschreibung: Human fetal lung
• Wachstumsmodus: Adherent
• Karyotyp: 2n=46, diploid
• Morphologie: Fibroblast-like
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human foetal lung

Beschreibung der Zelllinie

Established from normal lung tissue of a 14 week old male foetus. The cells undergo between 60-70 population doublings before senescence. The virus susceptibility of this line to human viruses is similar to WI-38.

DNA-Profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 9,11
D5S818: 11,12
D7S820: 10,11
THO1: 8
TPOX: 8
vWA: 15

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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