MRC-5 SV2

84100401, human lung (fetal), Fibroblast

• Synonym(e): MRC-5V2 Cells, MRC5V2 Cells
• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: MRC-5 SV2, 84100401
• Biologische Quelle: human lung (fetal)
• Wachstumsmodus: Adherent
• Karyotyp: Modal no. = 60-80, hyperdiploid
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human foetal lung, SV40 transformed

Beschreibung der Zelllinie

Derived from the parental cell line MRC-5 by transformation with the SV40 virus. The cells do not senesce.

Anwendung

Transformation and virus studies: SV40

DNA-Profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 11
D5S818: 11,12
D7S820: 10
THO1: 8
TPOX: 8
vWA: 15

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.