C1999

CMP-Sialic Acid Synthetase from Neisseria meningitidis group B

recombinant, expressed in E. coli BL21, ≥10 units/mg protein

• Synonym(e): CTP: N-Acylneuraminate cytidylyltransferase
• EC-Nummer: 2.7.7.43 (BRENDA, IUBMB)
• MDL-Nummer: MFCD01323074
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Rekombinant: expressed in E. coli BL21
• Qualitätsniveau: 200
• Form: lyophilized solid
• Spezifische Aktivität: ≥10 units/mg protein
• Mol-Gew.: 26.0 kDa
• Versandbedingung: dry ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Cytidine monophosphate (CMP)-Sialic Acid Synthetase from Neisseria meningitidis group B is encoded in neuA gene. The protein has a molecular weight of 24.8 kDa.

Anwendung

The enzyme has been utilized to synthesize CMP-sialic acid and its derivatives.

Biochem./physiol. Wirkung

Cytidine monophosphate (CMP)-sialic acid synthetase catalyses the conversion of N?acetylneuraminic acid (NeuNAc) to CMP-NeuNAc. CMP-sialic acid synthetase has globular α/β domain and is categorised under αβα three-layered sandwich fold. The dimerization domain aids the interaction between the monomers. It also has mononucleotide binding and NeuAc binding pocket. Mg²⁺ is essential for the catalytic functionality of CMP-sialic acid synthetase.

Einheitendefinition

One unit will catalyze the formation of 1 μmol CMP-Neu-5-Ac from Neu-5-Ac and CTP per minute at 37 °C at pH 8.0.

Physikalische Form

Supplied as a lyophilized powder containing Tris-HCl and NaCl.

Hinweis zur Analyse

Enzymatic activity assays are performed in Tris-HCl buffer (100 mM, pH 8.5) containing Neu-5-Ac (1 mM) and CTP (1 mM) at 37 °C for 30 min and analyzed using capillary electrophoresis with a UV detector (200 nm).

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable