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Wichtige Dokumente
I1643
Pyrophosphatase, anorganisch aus Backhefe (S. cerevisiae)
powder, ≥500 units/mg protein (E1%/280)
Synonym(e):
Anorganische Pyrophosphatase aus E. coli, Pyrophosphate phosphohydrolase
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About This Item
Empfohlene Produkte
Form
powder
Qualitätsniveau
Spezifische Aktivität
≥500 units/mg protein (E1%/280)
Mol-Gew.
71 kDa
Lagertemp.
−20°C
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Allgemeine Beschreibung
pI: 4.75
Anwendung
Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PPi + H2O → 2Pi.
It plays an important role in protein, RNA, and DNA synthesis.
It plays an important role in protein, RNA, and DNA synthesis.
Pyrophosphatase has been used:
- as a component of the reaction buffer used in aminoacylation assay to quantify incorporation of [3H]L-methionine into tRNA.
- as a component of PCR mixture.
Pyrophosphatase has been used:
as a component of the reaction buffer used in aminoacylation assay to quantify incorporation of [3H]L-methionine into tRNA.
as a component of PCR mixture
as a component of the reaction buffer used in aminoacylation assay to quantify incorporation of [3H]L-methionine into tRNA.
as a component of PCR mixture
Einheitendefinition
One unit will liberate 1.0 μmole of inorganic orthophosphate per min at pH 7.2 at 25 °C.
Physikalische Form
Lyophilized powder containing 80-90% buffer salts
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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Virginia P Ronchi et al.
The Journal of biological chemistry, 292(44), 18006-18023 (2017-09-20)
To understand the mechanism for assembly of Lys
Enzymatic chemiluminescence assay for inorganic pyrophosphate.
Vuokko Jansson et al.
Analytical biochemistry, 304(1), 135-137 (2002-04-24)
Laura Pedró-Rosa et al.
Journal of biomolecular screening, 20(1), 122-130 (2014-08-29)
Improved therapies for the treatment of Trypanosoma brucei, the etiological agent of the neglected tropical disease human African trypanosomiasis, are urgently needed. We targeted T. brucei methionyl-tRNA synthetase (MetRS), an aminoacyl-tRNA synthase (aaRS), which is considered an important drug target
Jin He et al.
Annals of neurology, 93(2), 244-256 (2022-09-12)
Despite the increasing number of genes associated with Charcot-Marie-Tooth (CMT) disease, many patients currently still lack appropriate genetic diagnosis for this disease. Autosomal dominant mutations in aminoacyl-tRNA synthetases (ARSs) have been implicated in CMT. Here, we describe causal missense mutations
Yibo Li et al.
Nature genetics, 46(4), 398-404 (2014-03-19)
Grain chalkiness is a highly undesirable quality trait in the marketing and consumption of rice grain. However, the molecular basis of this trait is poorly understood. Here we show that a major quantitative trait locus (QTL), Chalk5, influences grain chalkiness
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