A8481

Anti-γ-Actin-Antikörper, Maus monoklonal in Maus hergestellte Antikörper

clone 2-2.1.14.17, purified from hybridoma cell culture

• Synonym(e): Anti-ACTG1
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: purified from hybridoma cell culture
• Antikörper-Produkttyp: primary antibodies
• Klon: 2-2.1.14.17, monoclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~43 kDa
• Speziesreaktivität: mouse, chicken, canine, bovine, human, hamster
• Verpackung: antibody small pack of 25 μL
• Konzentration: ~2 mg/mL
• Methode(n): immunohistochemistry: suitable; indirect ELISA: suitable; western blot: 0.25-0.5 μg/mL using total cell extract of 3T3 cells
• Isotyp: IgG1
• UniProt-Hinterlegungsnummer: P63261
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: bovine ... ACTG1(404122) ; chicken ... ACTG1(415296) ; human ... ACTG1(71) ; mouse ... Actg1(11465)

Beschreibung

Allgemeine Beschreibung

γ-Actin is mainly present in sarcolemma and to a lesser extent in reticular lattice of normal muscles. Increased levels of γ-actin have been linked to dystrophin loss and subsequent cell signaling and gene expression defects.

Monoclonal Anti-γ-Actin (mouse IgG1 isotype) is derived from the hybridoma 2-2.1.14.17 produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice. Actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms. Actin isoforms show more than 90% overall sequence homology, but only 50-60% homology in their 18 NH₂-terminal residues. Actin constitutes for around 15% of the total protein. It exists in globular and filamentous forms.

Spezifität

Monoclonal Anti-γ-Actin recognizes human, bovine, canine, mouse, hamster and chicken γ-actin.

Anwendung

Anti-γ-Actin antibody, Mouse monoclonal has been used in :

• immunofluorescence
• enzyme linked immunosorbent assay (ELISA)
• western blotting
• immunohistochemistry

Mouse monoclonal Anti-γ-Actin antibody has been used for immunofluorescence assays. The antibody can also be used for ELISA, IHC and western blot assays.

Protein lysates from human Jurkat T-leukemic cells were subjected to western blot and probed using monoclonal mouse anti-actin (AC-40) to ensure equal loading.

Biochem./physiol. Wirkung

The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin is significantly involved in the microfilament system. It participates in muscle contraction and gene regulation. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Increased levels of γ-actin have been linked to dystrophin loss and subsequent cell signaling and gene expression defects.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Sonstige Hinweise

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)