A6941

Alkohol-Oxidase aus Candida boidinii

lyophilized powder, 5-15 units/mg protein

• Synonym(e): Alcohol:oxygen oxidoreductase
• CAS-Nummer: 9073-63-6
• EC-Nummer: 1.1.3.13 (BRENDA, IUBMB)
• EG-Nummer: 232-971-3
• MDL-Nummer: MFCD00130452
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: fungus (Candida boidinii)
• Qualitätsniveau: 200
• Form: lyophilized powder
• Spezifische Aktivität: 5-15 units/mg protein
• Mol-Gew.: octomer 600 kDa by sedimentation equilibrium
• Löslichkeit: 100 mM potassium phosphate, pH 7.5: soluble 1.0 mg/mL at 25 °C (Cold)
• Lagertemp.: −20°C

Beschreibung

Anwendung

Alcohol oxidase is used to catalyze the oxidation of short-chain, primary, aliphatic alcohols to their respective aldehydes . It may be used to study methanol metabolism is yeasts, such as Candida, Pichia, and Hansenula. It is useful to study protein translocation into peroxisomes. Product A6941 is from Candida boidinii and is provided as a lyophilized powder.

Biochem./physiol. Wirkung

Alcohol oxidase has the highest affinity for methanol. The affinity decreases with increasing chain length of the alkyl (R) group. Alcohol oxidase is primarily localized in the peroxisome but is also found in the cytoplasm. Alcohol oxidase is a 600 kDa homooctomeric flavoprotein with eight equal 74 kDa subunits; each containing a flavin adenine dinucleotide (FAD) molecule. The enzyme shows little activity toward secondary, tertiary, or aromatic alcohols; or aliphatic alcohols with a chain length of more than 5 carbons. The pH range for activity of this product is 6.5-8.5, with the optimum pH being 7.5.

Einheitendefinition

1 U oxidiert 1.0 μmol Methanol zu Formaldehyd pro Minute bei pH 7.5 und 25°C.

Physikalische Form

Enthält Kaliumphosphat-Puffersalze, DTE und Stabilisator

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)