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Merck

75164

Sigma-Aldrich

4-Methylumbelliferyl-Oleat

lipase substrate, ≥95% (HPCE), solid, suitable for fluorescence

Synonym(e):

4-Methylumbelliferyl-oleat

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About This Item

Empirische Formel (Hill-System):
C28H40O4
CAS-Nummer:
Molekulargewicht:
440.61
Beilstein:
4887028
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352204
PubChem Substanz-ID:
NACRES:
NA.21

product name

4-Methylumbelliferyl-Oleat, suitable for fluorescence, ≥95% (HPCE)

Qualitätsniveau

Assay

≥95% (HPCE)

Form

solid

Lagerbedingungen

protect from light
under inert gas

mp (Schmelzpunkt)

37-38 °C (lit.)

Löslichkeit

2-methoxyethanol: soluble
DMSO: soluble
chloroform: soluble

Fluoreszenz

λex 312 nm in methanol
λex 327 nm; λem 449 nm in 0.1 M phosphate pH 7.0 (lipase)

Eignung

suitable for fluorescence

Versandbedingung

wet ice

Lagertemp.

−20°C

SMILES String

CCCCCCCC\C=C/CCCCCCCC(=O)Oc1ccc2C(C)=CC(=O)Oc2c1

InChI

1S/C28H40O4/c1-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18-27(29)31-24-19-20-25-23(2)21-28(30)32-26(25)22-24/h10-11,19-22H,3-9,12-18H2,1-2H3/b11-10-

InChIKey

NKQFKJYKCVDLPT-KHPPLWFESA-N

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Anwendung

4-Methylumbelliferyl oleate has been used:
  • as a substrate to assay the activity of non-specific esterases
  • to determine lipase activity
  • as a substrate to evaluate pancreatic lipase inhibitory activity

Verpackung

Bottomless glass bottle. Contents are inside inserted fused cone.

Sonstige Hinweise

Fluorogenic substrate for lipase

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

M L Armstrong et al.
The American journal of pathology, 125(2), 339-348 (1986-11-01)
The authors studied xanthomatous skin in cholesterol-fed rabbits for changes in lipid content and in activities of enzymes regulating intracellular lipid content. After 80 days of hypercholesterolemic diet, xanthomas were widespread and changes in lipid metabolism were marked. In both
Oleate lipase activity in Gardnerella vaginalis and reconsideration of existing biotype schemes
Moncla BJ and Pryke KM
BMC Microbiology, 9(1), 78-78 (2009)
Lysosomal acid lipase in cultivated fibroblasts: characterization of enzyme activity in normal and enzymatically deficient cell lines.
B K Burton et al.
Clinica chimica acta; international journal of clinical chemistry, 101(1), 25-32 (1980-02-14)
Fluorometric assay for the hydrolytic activity of lipase using fatty acyl esters of 4-methylumbelliferone.
T J Jacks et al.
Analytical biochemistry, 21(2), 279-285 (1967-11-01)
T J Van Berkel et al.
Biochimica et biophysica acta, 617(3), 446-457 (1980-03-21)
(1) Parenchymal and non-parenchymal cells were isolated from rat liver. The characteristics of acid lipase activity with 4-methylumbelliferyl oleate as substrate and acid cholesteryl esterase activity with cholesteryl[1-14C]oleate as substrate were investigated. The substrates were incorporated in egg yolk lecithin

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