41658
Lipase-A-Candida antarctica, auf Immobead 150 immobilisiert, rekombinant aus Aspergillus oryzae
≥500 U/g
Synonym(e):
Candida antarctica Lipase
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About This Item
UNSPSC-Code:
12352204
NACRES:
NA.54
Empfohlene Produkte
Rekombinant
expressed in Aspergillus oryzae
Qualitätsniveau
Form
beads (powder like)
beads
Spezifische Aktivität
≥500 U/g
Lagertemp.
2-8°C
Allgemeine Beschreibung
Research area: Cellsignalling. Lipase A Candida Antarctica, CalA is a thermostable, calcium-dependent enzyme with high substrate specificity. CalA comprises of the catalytic triad (Ser184, Asp334, His366) and has an α/β hydrolase structural fold.
Anwendung
Lipase A Candida antarctica immobilized on Immobead 150, recombinant from Aspergillus oryzae has been used in the synthesis of enantiopure (R)-salsolinol and adsorption kinetics studies using Quartz crystal microbalance with dissipation (QCM-D).It has also been used tostudy the esterification of difluorinated alcohols.
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.
Biochem./physiol. Wirkung
Lipase A Candida Antarctica, CalA is highly specific for alcohols and esterifies the trans-isomer of fatty acids. CalA recognizes highly branched acyl groups and is active on alcohols with steric hindrance. CalA catalyzes the production of enantiopure amino acids and aids in the synthesis of chiral cyanohydrins. It may find industrial applications for its thermostable functionality in paper industry.Lipase A Candida antarctica(CAL-A) shows acetyltransferase activity by synthesizing fatty acid esters from certain alcohols and natural oils in an aqueous environment.
Einheitendefinition
1 U entspricht der Enzymmenge, die 1 μmol Buttersäure pro Minute bei pH 10.0 und 40°C freisetzt (Tributyrin, Fluka®-Nr. 91010, als Substrat)
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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High yield expression of Lipase A from Candida antarctica in the methylotrophic yeast Pichia pastoris and its purification and characterisation
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