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14479

Sigma-Aldrich

Phalloidin-Atto 490LS

suitable for fluorescence

Synonym(e):

Atto 490LS-Phalloidin

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About This Item

UNSPSC-Code:
12352200
NACRES:
NA.32

Assay

≥75.0% (HPLC)

Qualitätsniveau

Mol-Gew.

Mw 1466 g/mol

Hersteller/Markenname

ATTO-TEC GmbH

λ

in acetonitrile: water (1:1)

UV-Absorption

λ: 495-501 nm Amax

Eignung

suitable for fluorescence

Lagertemp.

−20°C

Allgemeine Beschreibung

Atto 490LS is a new fluorescent label featuring an extraordinary large Stokes-Shift of 165 nm. Thus the emission spectrum is almost completely separated from its absorption spectrum, making the dye highly suitable for multiplexing experiments, in particular in combination with Atto 488 and Atto 514. Atto 490LS is very hydrophillic and shows excellent water solubility. The dye exhibits a relatively high fluorescence quantum yield, which is only slightly reduced after conjugation to biomolecules, e.g. proteins, even at high degrees of labeling (DOL). Atto 490LS is an anionic dye. After conjugation to a substrate the dye carries a net electrical charge of -1.
Phalloidin, a bicyclic heptapeptide, is a very strong binding reagent to actin. Fluorescent labeled phalloidin has become a useful tool to investigate the distribution of F-actin within the cytoskeleton of cells by fluorescence microscopy.

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Rechtliche Hinweise

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Xiaomeng Zhang et al.
Oncogene, 39(30), 5267-5281 (2020-06-21)
Melanoma is a deadly form of skin cancer that accounts for a disproportionally large proportion of cancer-related deaths in younger people. Compared with most other skin cancers, a feature of melanoma is its high metastatic capacity, although the mechanisms that

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