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10108987001

Roche

Pyrophosphatase, inorganic (PPase)

from yeast

Synonym(e):

inorganic pyrophosphatase

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About This Item

EC-Nummer:
3.6.1.1 (BRENDA, IUBMB)
UNSPSC-Code:
12352204

Biologische Quelle

yeast

Qualitätsniveau

100

Form

suspension

Spezifische Aktivität

~200 units/mg protein (At 25 °C with inorganic pyrophosphate as the substrate.)

Verpackung

pkg of 1 mL (1 mg)

Hersteller/Markenname

Roche

Lagertemp.

2-8°C

Allgemeine Beschreibung

Pyrophosphate phosphohydrolase
The enzyme pyrophosphatase is ubiquitously distributed in a number of organisms.

Spezifität

Specific activity: Approximately 200 U/mg at +25°C with inorganic pyrophosphate as the substrate.

Anwendung

Inorganic pyrophosphatase has been used in enzyme assays and enzyme inhibition studies.
Pyrophosphatase, inorganic (PPase) has been used in :
  • Enzymatic nitric oxide (NO) detection system
  • In vitro transcription
  • Multiply-primed rolling circle amplification

Biochem./physiol. Wirkung

Inorganic pyrophosphatase catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate.
Mg ions are necessary for catalytic activity of crystalline pyrophosphatase.

Qualität

Contaminants: <0.01% ATPase and phosphatases, each (at pH 7 and 4-nitrophenyl phosphate as the substrate).

Physikalische Form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

No data available

Flammpunkt (°C)

No data available

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Michael A Kron et al.
Protein science : a publication of the Protein Society, 17(6), 983-989 (2008-04-16)
Diagnosis of eukaryotic parasitic infection using antibody-based tests such as ELISAs (enzyme-linked immunosorbent assays) is often problematic because of the need to differentiate between homologous host and pathogen proteins and to ensure that antibodies raised against a peptide will also
Tracking the m7G-cap during translation initiation by crosslinking methods.
Gross L, et al.
Methods, 137, 3-10 (2018)
2'-O-methylation of the mRNA cap protects RNAs from decapping and degradation by DXO.
Picard-Jean F, et al.
PLoS ONE, 13(3), e0193804-e0193804 (2018)
Yakov Y Woldman et al.
Free radical biology & medicine, 47(10), 1339-1345 (2009-09-16)
Nitric oxide (NO) is a free radical involved in many physiological processes including regulation of blood pressure, immune response, and neurotransmission. However, the measurement of extremely low, in some cases subnanomolar, physiological concentrations of nitric oxide presents an analytical challenge.
Single prokaryotic cell isolation and total transcript amplification protocol for transcriptomic analysis.
Kang Y, et al.
Nature Protocols, 10(7), 974-974 (2015)
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