N2415

Nukleosidphosphorylase bacterial

recombinant, expressed in E. coli, ≥10 units/mg protein

• Synonym(e): PNP, Purinnukleosid:Orthophosphatribosyltransferase, Purinnukleosidphosphorylase
• CAS-Nummer: 9030-21-1
• EC-Nummer: 2.4.2.1 (BRENDA, IUBMB)
• EG-Nummer: 232-857-3
• MDL-Nummer: MFCD00131739
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Rekombinant: expressed in E. coli
• Qualitätsniveau: 200
• Assay: ≥80% protein basis (biuret)
• Form: lyophilized powder
• Spezifische Aktivität: ≥10 units/mg protein
• Hinweis zur Sequenz: MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVRGMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLRDVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLFYSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAERQTTFNDMIKIALESVLLGDKE
• UniProt-Hinterlegungsnummer: P0ABP8
• Lagertemp.: −20°C
• SMILES String: [n]2(c3ncncc3nc2)[C@@H]1O[C@@H]([C@H]([C@H]1O)O)CO
• InChI: 1S/C10H12N4O4/c15-2-6-7(16)8(17)10(18-6)14-4-13-5-1-11-3-12-9(5)14/h1,3-4,6-8,10,15-17H,2H2/t6-,7-,8-,10-/m1/s1
• InChIKey: MRWXACSTFXYYMV-FDDDBJFASA-N
• Angaben zum Gen: Escherichia coli ... deoD(945654)

Beschreibung

Anwendung

Nukleosidphosphorylase wird in gekuppelten Enzymsystemen zum Messen der Proteindephosphorylierung verwendet.

Bacterial nucleoside phosphorylase has been used in a study that identified and characterized two adenosine phosphorylase activities in Mycobacterium smegmatis. Bacterial nucleoside phosphorylase has also been used in a study to investigate the inhibitionof pyrimidine and purine nucleoside phosphorylases by a 3,5-dichlorobenzoyl-substituted 2-deoxy-D-ribose-1-phosphate derivative.

Nucleoside Phosphorylase bacterial has been used in reaction buffer and buffer-exchanged to remove residual phosphate.

Biochem./physiol. Wirkung

Katalysiert die folgende Reaktion:Purinnukleosid + Phosphat = Purin + alpha-D-Ribose-1-Phosphat

Nucleoside Phosphorylase/purine nucleoside phosphorylase (PNP), an enzyme participates in the purine salvage pathway. It also helps in the reversible cleavage of inosine to hypoxanthine and guanosine to guanine. PNP deficiency results in neurologic manifestations.

Einheitendefinition

One unit will cause the phosphorolysis of 1.0 micromole of inosine to hypoxanthine andribose-1-phosphate per min at pH 7.4 at 25 deg C.

Angaben zur Herstellung

This is a salt-free and animal component-free enzyme preparation. This enzyme should be solubilized in a 10mM KPO₄, pH 7.4 solution.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable