10108014001

Papain

from Carica papaya

• Synonym(e): cystein protease, papain
• EC-Nummer: 3.4.22.2 (BRENDA, IUBMB)
• UNSPSC-Code: 12352204

Eigenschaften

• Biologische Quelle: papaya
• Qualitätsniveau: 100
• Form: suspension
• Spezifische Aktivität: 30 U/mg; ~30 units/mg protein (At 25 °C with BAEE as the substrate.)
• Mol-Gew.: 23 kDa
• Verpackung: pkg of 10 mL (100 mg)
• Hersteller/Markenname: Roche
• Konzentration: 10-100 mg/mL
• Optimaler pH-Wert: 6.0-7.0
• Versandbedingung: wet ice

Beschreibung

Allgemeine Beschreibung

Papain is a cysteine protease of the papain family. It is a single chain protein. The molecular weight of papain is 23 kDa. Papain is composed of two structural domains and a cleft between them, which consists of the active site.

Anwendung

Papain has been used in the digestion of various brain cells.

Use Papain for the complete proteolytic cleavage of proteins, limited hydrolysis of native immunoglobulins, and tissue dissociation (together with collagenase, esterase, and trypsin). The enzyme solubilizes integral membrane proteins and produces glycopeptides from purified proteoglycans. The addition of cysteine (approximately 0.5% [w/v]) is essential for enzyme activity.

Biochem./physiol. Wirkung

Papain exhibits proteolytic activity against amide links, amino acid esters, peptides, proteins. It mainly cleaves peptide bonds containing amino acids such as lysine, arginine and residues succeeding phenylalanine. It can catalyze several reactions, such as hydrolysis, transferase action, specificity and acyl-enzyme intermediate.

Leistungsmerkmale und Vorteile

Inhibitors: SH-blocking reagents, iodoacetic acid, iodoacetamide, TPCK, TLCK, leupeptin, a2-macroglobulin, E-64, PMSF, and antipain, Hg2+, and other heavy metals

Physikalische Form

Suspension, crystalline, nonsterile

Angaben zur Herstellung

Working concentration: 0.05 to 0.5 mg/ml

Lagerung und Haltbarkeit

Store at 2 to 8 °C. (a decrease in activity of 20% may occur within 6 months)

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: nwg
• Flammpunkt (°F): does not flash
• Flammpunkt (°C): does not flash