MABS1950

Anti-p15 Antibody, clone PAT56B

clone PAT56B, from rat

• Synonym(e): Cyclin-dependent kinase 4 inhibitor B, p14-INK4b, p15-INK4b
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rat
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: PAT56B, monoclonal
• Speziesreaktivität: mouse
• Verpackung: antibody small pack of 25 μg
• Methode(n): immunohistochemistry: suitable; western blot: suitable
• Isotyp: IgG2aκ
• NCBI-Hinterlegungsnummer: NP_031696.1
• UniProt-Hinterlegungsnummer: P55271
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: mouse ... Cdkn2B(12579)

Beschreibung

Allgemeine Beschreibung

Cyclin-dependent kinase 4 inhibitor B (UniProt: P55271; also known as p14-INK4b, p15-INK4b) is encoded by the Cdkn2b gene (Gene ID: 12579) in murine species. p15-INK4b is a ubiquitously expressed member of the INK4 family of cyclin-dependent kinase inhibitors. It induces a G1-phase cell cycle arrest through heterodimerization with cyclin-dependent kinase 4/6, thereby serves as a tumor suppressor. It also serves as a potential effector of TGF-beta induced cell cycle arrest. p15 also plays an important role in the regulation of cellular commitment of hematopoietic progenitor cells and myeloid cell differentiation. It contains four ankyrin repeats (aa 5-34; 38-66; 71-100; and 104-130). p15 shares extensive homology with p16 and serves an important backup function for p16 in senescence. Cdkn2b gene is frequently hypermethylated in myeloid neoplasia. Genes coding for both p15 and p16 are reported to often deleted in human tumors. p15 can serve as a good prognostic marker for monitoring the response to treatment with DNA methylation inhibitors. (Ref.: Wolff, L., and Bies, J. (2013). Blood Cells Mol. Dis. 50(4); 227-231).

Spezifität

Clone PAT56B is a rat monoclonal antibody that specifically detects murine Cyclin-dependent kinase 4 inhibitor B (p15-INK4b).

Immunogen

GST/His-tagged recombinant full-length mouse Cyclin-dependent kinase 4 inhibitor B (p15-INK4b).

Anwendung

Research Category
Zelluläre Signaltransduktion

Anti-p15, clone PAT56B, Cat. No. MABS1950, is a rat monoclonal antibody that detects Cyclin-dependent kinase 4 inhibitor B (p15-INK4b) and has been tested for use in Immunohistochemistry and Western Blotting.

Immunohistochemistry Analysis: A representative lot detected p15 in Immunohistochemistry applications (Munoz-Espin, D., et. al. (2013). Cell. 155(5):1104-18).

Qualität

Evaluated by Western Blotting in NIH/3T3 cell lysates.

Western Blotting Analysis: 4 µg/mL of this antibody detected p15 in NIH/3T3 cell lysates.

Zielbeschreibung

~14 kDa observed; 13.79 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physikalische Form

Protein G purified

Format: Purified

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable