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MABS1289

Sigma-Aldrich

Anti-T4 Lysozyme (T4L) Antibody, clone 6A6

clone 6A6, from mouse

Synonym(e):

Bacteriophage T4 endolysin, Bacteriophage T4 lysis protein, Bacteriophage T4 lysozyme, Bacteriophage T4 muramidase

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

6A6, monoclonal

Speziesreaktivität

phage T4

Speziesreaktivität (Voraussage durch Homologie)

all

Methode(n)

immunoprecipitation (IP): suitable
western blot: suitable

Isotyp

IgG1κ

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

ambient

Posttranslationale Modifikation Target

unmodified

Allgemeine Beschreibung

Bacteriophage T4 endolysin (EC 3.2.1.17; UniProt P00720; also known as T4 lysis protein, T4 lysozyme, T4 muramidase, T4L) is encoded by the T4 E gene. T4 lysozyme (T4L) is a 164-a.a. monomeric protein that hydrolyzes peptidoglycan.T4L functions outside the cytoplasmic membrane and starts the phage extracellular phase by liberating virions from the metabolically inert remains of the bacterial host. Similar to glutathione S-transferase (GST) and T7 lysozyme, T4L sequence is commonly utilized to generate soluble recombinant fusions of hydrophobic proteins, such as multitransmembrane proteins, that are otherwise not soluble when expressed entirely in their native sequences. Antibodies against T4L are useful in detecting T4L in T4-infected bacteria hosts and in probing recombinant T4L fusion proteins.

Spezifität

Clone 6A6 detects T4L and recombinant T4L fusion proteins.

Immunogen

Recombinant T4L fusion protein.

Anwendung

Research Category
Epitop-Tags & allgemeine Anwendung
Anti-T4 Lysozyme (T4L) Antibody, clone 6A6, Cat. No. MABS1289, is a highly specific mouse monoclonal antibody that targets bacteriophage T4 lysozyme (T4L), validated for use in Immunoprecipitation and Western Blotting for the detection of T4L and T4L-tagged fusion proteins.
Immunoprecipitation Analysis: 1 µg from a representative lot immunoprecipitated exogenously expressed TL4 fusion protein from 1.5 µg of lysate of transfected cells by an independent laboratory.

Qualität

Western Blotting Analysis: 0.1 µg/mL of this antibody detected exogenously expressed T4L fusion protein in 0.2 µg of lysate from transfected cells.

Zielbeschreibung

18.69 kDa calculated.

Physikalische Form

Protein G purified.
Format: Purified
Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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