IP04
Protein G Plus-Agarose Suspension
Protein G PLUS agarose suspension specifically formulated for immunoprecipitation.
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About This Item
UNSPSC-Code:
41116133
NACRES:
NA.56
Empfohlene Produkte
Form
slurry (Liquid)
Enthält
≤0.1% sodium azide as preservative
Hersteller/Markenname
Calbiochem®
Lagerbedingungen
do not freeze
Methode(n)
immunoprecipitation (IP): suitable
Eignung
suitable for immunoprecipitation
Versandbedingung
wet ice
Lagertemp.
2-8°C
Allgemeine Beschreibung
Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification; best for bovine, goat, human, and rat IgG and for mouse IgG1.
Protein G PLUS agarose suspension specifically formulated for immunoprecipitation.
Warnhinweis
Toxicity: Standard Handling (A)
Sonstige Hinweise
Agarose solution is supplied ready to use. Protein G Plus agrarose is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl/µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl agarose conjugate and 1 µg normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
Rechtliche Hinweise
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
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