P2545

Protein A–Agarose

saline suspension

• Synonym(e): Protein A resin
• MDL-Nummer: MFCD00165563
• UNSPSC-Code: 41106500
• NACRES: NA.56

Eigenschaften

• Biologische Quelle: Staphylococcus aureus
• Qualitätsniveau: 200
• Form: saline suspension
• Kennzeichnungsgrad: ~2 mg per mL
• Matrixaktivierung: cyanogen bromide
• Matrixanbindung: amino
• Matrix-Spacer: 1 atom
• Kapazität: ≥15 mg/mL binding capacity (human IgG)
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Protein A is a 42-kDa protein from Staphylococcus aureus and has five immunoglobulin binding domains.

Anwendung

Protein A−Agarose has been used in the immunoprecipitation

• of protein phosphatase and tensin homolog (PTEN) protein from S63 and NIH3T3 fibroblast cells
• of prolyl hydroxylase domain 2 N27 cells
• of Wnt3a and sonic hedgehog (Shh) in mouse fibroblast L-cells and human embryonic kidney (HEK) 293 cells

Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins. Protein A-agarose has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.

Biochem./physiol. Wirkung

Protein A binds to Fcγ (the constant region) and Fab (antigen binding fragment) of IgG. Protein A agarose resin is commercially used for affinity based purifications.

Physikalische Form

Suspension in 0.5 M NaCl, enthält 0.02% Thiomersal

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)