AP501B

Goat Anti-Mouse IgM+IgG+IgA (H+L) Antibody, biotin conjugate, Species Adsorbed

Chemicon^®, from goat

• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.46

Eigenschaften

• Biologische Quelle: goat
• Qualitätsniveau: 100
• Konjugat: biotin conjugate
• Antikörperform: affinity purified immunoglobulin
• Antikörper-Produkttyp: secondary antibodies
• Klon: polyclonal
• Speziesreaktivität: mouse
• Hersteller/Markenname: Chemicon^®
• Methode(n): ELISA: suitable; immunofluorescence: suitable
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

IgG is the most abundant immunoglobulin compose of two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.

Spezifität

Reacts with heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA. Absorbed for human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins. Reactivity with other species has not been determined.

The antibody reacts with the heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.

Immunogen

Epitope: Mouse IgM, IgG, IgA

Prepared from pooled antisera from goats hyperimmunized with mouse IgM, IgG and IgA paraproteins.

Anwendung

Research Category
Sekundär- & Kontrollantikörper

Research Sub Category
Sekundärantikörper zur Doppelmarkierung

Detect Mouse IgM+IgG+IgA (H+L) using this Goat anti-Mouse IgM+IgG+IgA (H+L) Antibody, biotin conjugate, Species Adsorbed validated for use in ELISA & IF.

Immunofluorescence: ≤1 μg/10E6 cells
ELISA: 1:5,000-1:20,000

Optimal working dilutions must be determined by the end user.

Physikalische Form

Affinity Chromatography

Purified by affinity chromatography on pooled mouse IgM+IgG+IgA covalently linked to agarose. Liquid in 2.0 mL PBS/NaN3.

Lagerung und Haltbarkeit

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable