654245

TNF-α, Mouse, Recombinant, E. coli

• Synonym(e): rmTNF-α, rm-Cachectin, Tumor Necrosis Factor-α
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Rekombinant: expressed in E. coli
• Assay: ≥97% (SDS-PAGE)
• Form: lyophilized
• Mol-Gew.: 17,000 g/mol
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Methode(n): cell based assay: suitable
• Verunreinigungen: ≤100 pg/μg Endotoxin (pg/μg TNF-α)
• Versandbedingung: ambient
• Lagertemp.: −70°C
• Angaben zum Gen: mouse ... Tnf(21926)

Beschreibung

Allgemeine Beschreibung

Reasearch area: Apoptosis

Recombinant mouse tumor necrosis factor-α (TNF-α), expressed in E. coli, is known for its direct tumoricidal capabilities. This inflammatory cytokine triggers a cascade of other cytokines and factors constituting the immune system′s response to infections and cancer. Additionally, it has been reported to influence changes in bone metabolism during inflammation. (TNF-α), a homotrimer protein, occurs in a soluble and transmembrane form.

Anwendung

TNF-α, Mouse, Recombinant, E. coli has been used to study TNF-signaling to insulin gene expression in insulin-producing cells.

Biochem./physiol. Wirkung

TNF-α, a cytokine, interacts with two distinct receptors, initiating signal transduction pathways that result in cellular responses, such as cell survival, differentiation, and proliferation. It regulates inflammatory responses and activates inflammatory molecules, including other cytokines and chemokines. Transmembrane TNF-α binds to TNFR1 and TNFR2 and facilitates inflammation and cell death. However, excessive or inappropriate activation of TNF-α signaling is linked to chronic inflammation and may lead to pathological complications, including autoimmune diseases. Rheumatoid arthritis (RA), inflammatory bowel disease (IBD), psoriatic arthritis (PsA), psoriasis (PS), and noninfectious uveitis (NIU) are few conditions.

Additionally, TNF-α is associated with insulin signaling inhibition. Its removal ameliorates insulin sensitivity. The activity of the Jun N-terminal kinase (JNK) signaling pathway, which is abnormally elevated in obesity, is strongly activated by both TNF-α.

ED₅₀ = 20-50 pg/ml as measured in a cytotoxic assay using the TNF-α susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

Lyophilized from sterile-filtered PBS, 50 µg BSA/µg TNF-α.

Rekonstituierung

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.

Reconstitute to a concentration ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.

Sonstige Hinweise

Scharla, S.H., et al. 1994. Eur. J. Endocrinol. 131, 293.
Eck, M.J., et al. 1992. J. Biol. Chem. 267, 2119.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable