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05-366

Sigma-Aldrich

Anti-PLCγ-1 Antibody, clone B-6-4

clone B-6-4, 1 mg/mL, Upstate®

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

B-6-4, monoclonal

Speziesreaktivität

human, rabbit, rat, bovine, mouse

Hersteller/Markenname

Upstate®

Konzentration

1 mg/mL

Methode(n)

immunoprecipitation (IP): suitable
western blot: suitable

Isotyp

IgG1

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... PLCG1(5335)

Spezifität

PLCγ-1; may also react with some non-PLC γ-1 SH-3 containing proteins

Immunogen

Purified soluble phospholipase C (PLC) g-1 from bovine brain

Anwendung

Research Category
Zelluläre Signaltransduktion
Research Sub Category
Lipidabhängige Signalübertragung
Anti-PLCγ-1 Antibody, clone B-6-4 is an antibody against PLCγ-1 for use in IP & WB.

Qualität

Routinely evaluated in immunoblot using acid precipitated protein from bovine brain, RIPA lysates from human A431 cells, and previously using mouse 3T3/A31 fibroblasts and rat L6 myoblasts lysates.

Zielbeschreibung

~135 kDa

Physikalische Form

Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1 antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Piktogramme

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Signalwort

Warning

Gefahreneinstufungen

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1


Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

L Rui et al.
The Journal of biological chemistry, 274(15), 10590-10594 (1999-04-03)
Nerve growth factor (NGF) is essential for the development and survival of sympathetic and sensory neurons. NGF binds to TrkA, activates the intrinsic kinase activity of TrkA, and promotes the differentiation of pheochromocytoma (PC12) cells into sympathetic-like neurons. Several signaling
T Fukazawa et al.
The Journal of biological chemistry, 270(34), 20177-20182 (1995-08-25)
Tyrosine phosphorylation of cellular proteins is an early and an essential step in T cell receptor-mediated lymphocyte activation. Tyrosine phosphorylation of transmembrane receptor chains (such as zeta and CD3 chains) and membrane-associated proteins provides docking sites for SH2 domains of
Phospholipase C and src tyrosine kinases mediate neurotensin-stimulated Cl- secretion in rabbit proximal colon.
Roli Prasad, Jayashree Venkatasubramanian, Milen Amde, Mrinalini Chatta Rao
Digestive Diseases and Sciences null
Tyrosine phosphorylation and recruitment of ZAP-70 to the CD3-TCR complex are defective after CD2 stimulation
Hubert, P., et al
Journal of immunology (Baltimore, Md. : 1950), 157, 4322-4332 (1996)
CD3 stimulation causes phosphorylation of phospholipase C-gamma 1 on serine and tyrosine residues in a human T-cell line.
Park, D J, et al.
Proceedings of the National Academy of Sciences of the USA, 88, 5453-5456 (1991)

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