808512

γ-[(Propargyl)-imido]-ATP sodium salt

• Synonym(e): Adenosine-5’-[γ-(propargyl)-imido]triphosphate sodium salt
• Empirische Formel (Hill-System): C₁₃H₁₉N₆O₁₂P₃ · xNa⁺
• Molekulargewicht: 544.24 (free acid basis)
• UNSPSC-Code: 12352200
• PubChem Substanz-ID: 329769104
• NACRES: NA.22

Eigenschaften

• Assay: ≥95% (HPLC)
• Qualitätsniveau: 200
• Form: solid
• Eignung der Reaktion: reaction type: click chemistry
• Löslichkeit: 10 mM Tris-HCl, pH 7.5: soluble
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• SMILES String: NC1=NC=NC2=C1N=CN2[C@H]3O[C@H](COP(OP(O)(OP(O)(NCC#C)=O)=O)(O)=O)[C@@H](O)[C@H]3O
• InChI: 1S/C13H19N6O12P3/c1-2-3-18-32(22,23)30-34(26,27)31-33(24,25)28-4-7-9(20)10(21)13(29-7)19-6-17-8-11(14)15-5-16-12(8)19/h1,5-7,9-10,13,20-21H,3-4H2,(H,24,25)(H,26,27)(H2,14,15,16)(H2,18,22,23)/t7-,9-,10-,13?/m1/s1
• InChIKey: PIPOEUYSJJTELD-RJNFYWFKSA-N

Beschreibung

Anwendung

Lee, et al,. reported a non-radioactive version of in vitro phosphorylation where γ-[(Propargyl)-imido]-ATP (compound 1) was successfully used instead of γ-³²P-modified ATP to phosphorylate GST-tagged recombinant p27kip1 with protein kinase cdk2. The phosphorylated, alkyne-modified protein substrate can be labeled with azides of biotin or fluorescent dyes using Cu(I)-catalyzed click chemistry.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable