A2647

Adenosin-5′-(β,γ-imido)triphosphat Tetralithiumsalz Hydrat

≥93% (HPLC), powder

• Synonym(e): βγ-Imidoadenosin-5′-triphosphat Tetralithiumsalz, AMP-PNP, ATP[β,γ-NH], Adenylyl-imidodiphosphat Tetralithiumsalz, App(NH)p
• Empirische Formel (Hill-System): C₁₀H₁₇N₆O₁₂P₃ · xLi⁺ · yH₂O
• CAS-Nummer: 25612-73-1
• Molekulargewicht: 506.20 (free acid basis)
• Beilstein: 6047109
• UNSPSC-Code: 41106305
• PubChem Substanz-ID: 24890685
• NACRES: NA.51

Eigenschaften

• Biologische Quelle: natural (organic)
• Qualitätsniveau: 200
• Assay: ≥93% (HPLC)
• Form: powder
• Farbe: white
• Löslichkeit: H₂O: 50 mg/mL
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• SMILES String: [Li+].[Li+].[Li+].[Li+].[H]O[H].Nc1ncnc2n(cnc12)[C@@H]3O[C@H](COP([O-])(=O)OP([O-])(=O)NP([O-])([O-])=O)[C@@H](O)[C@H]3O
• InChI: 1S/C10H17N6O12P3/c11-8-5-9(13-2-12-8)16(3-14-5)10-7(18)6(17)4(27-10)1-26-31(24,25)28-30(22,23)15-29(19,20)21/h2-4,6-7,10,17-18H,1H2,(H,24,25)(H2,11,12,13)(H4,15,19,20,21,22,23)/t4-,6-,7-,10-/m1/s1
• InChIKey: PVKSNHVPLWYQGJ-KQYNXXCUSA-N

Beschreibung

Anwendung

Adenosine 5′-(β,γ-imido)triphosphate lithium salt hydrate has been used in a study to research the effects of monovalent cations Li⁺, Na⁺, K⁺, NH₄⁺, Rb⁺ and Cs⁺ on the solid and solution structures of the nucleic acid components. Adenosine 5′-(β,γ-imido)triphosphate lithium salt hydrate has also been used in a study to determine that antidepressants inhibit interferon-γ-induced microglial production of IL-6 and nitric oxide.

Biochem./physiol. Wirkung

A non-hydrolyzable ATP analog. AMP-PNP competitively inhibits ATP-dependent enzyme systems, such as glutamine synthetase. It inhibits a number of enzymes which require the hydrolysis of ATP such as DNA topoisomerase II, SV40 large T-antigen helicase and a number of kinases. Blocks ATP-sensitive calcium-dependent potassium channels.

Vorsicht

AMP-PNP is very unstable in acidic conditions; rapidly hydrolyzes to the phosphoramidate and inorganic phosphate.

Rekonstituierung

A 10 mg/mL stock solution may be made, aliquoted and stored at -70 °C for up to 3 months.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)