565324-U

Ascentis^® RP-Amide (5 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

• Synonyme(s) : RP-Amide Reversed-Phase Chromatography Column
• Code UNSPSC : 41115700
• eCl@ss : 32110501
• Nomenclature NACRES : SB.52

Propriétés

• product name: Colonne HPLC^® Ascentis RP-Amide, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
• Matériaux: stainless steel column
• Niveau de qualité: 100
• Agence: suitable for USP L60
• Gamme de produits: Ascentis^®
• Caractéristiques: endcapped
• Fabricant/nom de marque: Ascentis^®
• Conditionnement: 1 ea of
• Ampleur du marquage: 19.5% Carbon loading
• Paramètres: ≤70 °C temp. range; 400 bar pressure (5801 psi)
• Technique(s): HPLC: suitable; LC/MS: suitable
• L × D.I.: 15 cm × 4.6 mm
• Superficie: 450 m²/g
• Couverture de surface: 2.7 μmol/m²
• Impuretés: <5 ppm metals
• Matrice: fully porous particle; silica gel high purity, spherical
• Groupe de la matrice active: amide, alkyl phase
• Taille des particules: 5 μm
• Dimension de pores: 100 Å
• operating pH range: 2-8
• Application(s): food and beverages
• Technique de séparation: reversed phase

Description

Description générale

Ascentis RP-Amide est une phase EPG (groupement polaires inclus) de nouvelle génération à faible relargage, qui fournit une sélectivité orthogonale et une plus grande résolution pour l′analyse des composés polaires en HPLC et LC-MS. L′Ascentis RP-Amide la phase de première intention à groupement polaire incorporé pour HPLC.

Application

• Preparation of mixed-mode stationary phase for separation of peptides and proteins in high-performance liquid chromatography: Highlights the application of Ascentis^® RP-Amide HPLC column in the development of mixed-mode stationary phases, further demonstrating its versatility in handling diverse biochemical compounds and its pivotal role in advancing pharmaceutical and proteomic research (Alharthi et al., 2022).


• On-line hyphenation of solid-phase extraction to chromatographic separation of sulfonamides with fused-core columns in sequential injection chromatography: Showcases the efficacy of Ascentis^® RP-Amide HPLC columns in environmental analysis, specifically for the detection of contaminants in complex matrices. This study underscores the column′s robustness and high-resolution capabilities, making it invaluable in environmental laboratories (Batista et al., 2015).


• Determination of zearalenone and its metabolites in endometrial cancer by coupled separation techniques: Demonstrates the critical role of Ascentis^® RP-Amide HPLC columns in clinical research, particularly in the sensitive and accurate measurement of biomarkers in cancer diagnostics. The column′s superior retention for polar compounds proves essential for precise biomedical analyses (Gadzała-Kopciuch et al., 2011).

Caractéristiques et avantages

• Rétention et forme de pics excellentes pour les composés polaires
• Compatibilité aqueuse 100 %
• très faible relargahe, compatible LC-MS
• Sélectivité unique

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Informations légales

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany