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RAB0058

Sigma-Aldrich

Rat MCP-1 / CCL2 ELISA Kit

for cell and tissue lysates

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About This Item

Code UNSPSC :
41116158
Nomenclature NACRES :
NA.32

Espèces réactives

rat

Conditionnement

kit of 96 wells (12 strips x 8 wells)

Technique(s)

ELISA: suitable
capture ELISA: suitable

Entrée

sample type cell lysate
sample type tissue lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 15 pg/mL
standard curve range: 24.67-18000 pg/mL

Méthode de détection

colorimetric

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

rat ... Ccl2(24770)

Description générale

The Rat MCP-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat MCP-1 in cell lysate and tissue lysate.

Immunogène

Recombinant Rat MCP1

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Actions biochimiques/physiologiques

Chemokine (C-C motif) ligand 2 (CCL2) or monocyte chemoattractant protein-1 (MCP-1) is a pro-inflammatory factor expressed in astrocytes. It is synthesized by monocytes, B cells, fibroblast cells, endothelial and glial cells. CCL2 activates monocytes and regulates cytokine and adhesion molecule synthesis. The protein modulates neuropeptide function and development.

Autres remarques

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)FDS
  • RABLYSIS12X Cell Lysis Buffer (Item J)FDS
  • RABSTOP3ELISA Stop Solution (Item I)FDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)FDS
  • RABWASH420X Wash Buffer (Item B)FDS

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Warning

Mentions de danger

H290

Conseils de prudence

P234 - P390

Classification des risques

Met. Corr. 1

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Prenatal fat-rich diet exposure alters responses of embryonic neurons to the chemokine, CCL2, in the hypothalamus.
Poon K
Neuroscience (2016)
Dexmedetomidine Attenuates Lipopolysaccharide Induced MCP-1 Expression in Primary Astrocyte
BioMed Research International (2017)
Zai-Long Chi et al.
Neuropharmacology, 148, 96-106 (2018-12-31)
Neurodegenerative diseases affect millions of people worldwide. Optic neuropathies are the most commonly occurring neurodegenerative diseases, characterized by progressive retinal ganglion cell (RGC) degeneration. We recently reported that Prominin-1, a protein found on the surface of stem cells, interacts with
Ruozhi Zhao et al.
The Journal of nutritional biochemistry, 25(8), 851-857 (2014-06-14)
Atherosclerotic cardiovascular complications are the leading cause of death in diabetic patients. Monocyte adhesion is an early event for atherogenesis. Previous studies demonstrated that dark-skin berries had cardiovascular protective effects. We hypothesize that Saskatoon berry (SB) powder may reduce monocyte
John E S Park et al.
Thorax, 69(12), 1120-1127 (2014-09-17)
The burden of chronic heart failure (HF) is rising owing to an increased survivorship after myocardial infarction (MI). Pulmonary structural remodelling in patients with HF may protect against oedema while causing dyspnoea, the predominant symptom associated with HF. The cellular
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