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Key Documents

RAB0507

Sigma-Aldrich

Human VEGF-A ELISA Kit

for serum, plasma, cell culture supernatants and urine

Synonyme(s) :

VEGF-A, Vascular endothelial growth factor A 

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About This Item

Code UNSPSC :
41116158
Nomenclature NACRES :
NA.32

Espèces réactives

human

Conditionnement

kit of 96 wells (12 strips x 8 wells)

Technique(s)

ELISA: suitable
capture ELISA: suitable

Entrée

sample type plasma
sample type cell culture supernatant(s)
sample type serum
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 10 pg/mL
standard curve range: 8.23-6000 pg/mL

Méthode de détection

colorimetric

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... VEGFA(7422)

Description générale

The Human VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human VEGF in serum, plasma, cell culture supernatants and urine.

Immunogène

Recombinant Human VEGF-A

Application

For research use only. Not for use in diagnostic procedures.
Human VEGF (vascular endothelial growth factor) ELISA Kit has been used to measure VEGFA levels in human umbilical vascular endothelial cells (HUVECs) incubated with plasma from pre-eclamptic and healthy pregnant women.
Human VEGF-A ELISA Kit has been used to qualify:
  • VEGF in human liver hepatoma cell line PLC-PRF-5,
  • residual cytokines in decellularized meningeal scaffolds (DMS)
  • human periodontal ligament (hPDL) fibroblasts

Actions biochimiques/physiologiques

VEGF (vascular endothelial growth factor) is a potent angiogenic cytokine. It stimulates proliferation and migration of endothelial cells. VEGF induces microvascular permeability by enhancing function of vesicular-vacuolar organelle (VVO). VEGF plays a prominent role in both normal and pathological angiogenesis. Substantial evidence suggests that VEGF is involved in the induction of tumor metastasis and intraocular neovascular syndromes. Increased expression of VEGF causes retinal angiogenesis, thus, inhibition of VEGF using soluble VEGF-receptor chimeric proteins can be considered as a potential therapy for ischemic retinal disease. Polymorphism in VEGF at C (-634) G is linked with diabetic retinopathy in type 2 diabetes. Altered expression of VEGF is also associated with rheumatoid arthritis, Graves′ disease, psoriasis, cardiovascular disease, and peripheral limb disease.

Autres remarques

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)FDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)FDS

  • RABSTOP3ELISA Stop Solution (Item I)FDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)FDS

  • RABWASH420X Wash Buffer (Item B)FDS

Pictogrammes

Corrosion

Mention d'avertissement

Warning

Mentions de danger

Conseils de prudence

Classification des risques

Met. Corr. 1

Code de la classe de stockage

8A - Combustible corrosive hazardous materials


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Plasma from pre-eclamptic patients induces the expression of the anti-angiogenic miR-195-5p in endothelial cells.
Sandrim VC
Journal of Cellular and Molecular Medicine, 20(6), 1198-1200 (2016)
Immunomodulatory drugs disrupt the cereblon-CD147-MCT1 axis to exert antitumor activity and teratogenicity.
Eichner R
Nature Medicine, 22(7), 735-743 (2016)
The role of mechanotransduction versus hypoxia during simulated orthodontic compressive strain?an in vitro study of human periodontal ligament fibroblasts
Ullrich N, et al.
International Journal of Oral Science, 11(4), 1-10 (2019)
Yue Zhao et al.
Evidence-based complementary and alternative medicine : eCAM, 2020, 5204917-5204917 (2020-05-19)
To evaluate the efficacy of Abelmoschus manihot in treating type 2 diabetic nonproliferative retinopathy. It was a randomized controlled clinical trial. The recruited eighty subjects with type 2 diabetic nonproliferative retinopathy were randomly divided into treatment group and control group.
Exogenous hydrogen sulfide promotes hepatocellular carcinoma cell growth by activating the STAT3-COX-2 signaling pathway
Zhen Y, et al.
Oncology Letters, 15(5), 6562-6570 (2018)

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