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Key Documents

RAB0182

Sigma-Aldrich

Human bFGF ELISA Kit

for serum, plasma, cell culture supernatant and urine

Synonyme(s) :

Basic fibroblast growth factor, FGF-2, Fibroblast growth factor 2, HBGF-2, Heparin-binding growth factor 2, bFGF

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About This Item

Code UNSPSC :
41116158
Nomenclature NACRES :
NA.32

Espèces réactives

human

Conditionnement

kit of 96 wells (12 strips x 8 wells)

Technique(s)

ELISA: suitable
capture ELISA: suitable

Entrée

sample type cell culture supernatant(s)
sample type serum
sample type plasma
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 50 pg/mL
standard curve range: 102.4-10000 pg/mL

Méthode de détection

colorimetric

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... FGF2(2247)

Description générale

The Human bFGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human bFGF in serum, plasma, cell culture supernatants and urine.

Immunogène

Recombinant Human basic-FGF

Application

Human bFGF ELISA kit has been used:
  • to quantify the level of residual cytokines in decellularized meningeal scaffolds (DMS)
  • to measure cytokine levels in acellularized meningeal scaffolds (AMS) and recovered meningeal tissues (RMS/CMS)
  • to analyze basic-fibroblast growth factor (bFGF) concentration in cell culture medium

Actions biochimiques/physiologiques

FGF2 (fibroblast growth factor 2, also referred to as BFGF (basic fibroblast growth factor)) interacts with FGFR1 (fibroblast growth factor receptor 1), FGFR2 and FGFR3. It stimulates the proliferation of a wide variety of cells, including mesenchymal, neuroectodermal and endothelial cells. FGF2 also determines the fate of neural precursor cells and promotes neuronal axon regeneration. It plays an important role in spinal cord injury repair. FGF2 is known to stimulate angiogenesis and is associated with cancer development. High levels of FGF2 are present in the kidney of children with HIV (human immunodeficiency virus)-renal diseases. MicroRNA (miR)-503 targets FGF2 mRNA and inhibits migration and invasiveness of osteosarcoma (OS) cells.

Autres remarques

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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Réf. du produit
Description
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  • RABDIL6ELISA 5X Sample Diluent Buffer (Item D2)FDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)FDS
  • RABSTOP3ELISA Stop Solution (Item I)FDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)FDS
  • RABWASH420X Wash Buffer (Item B)FDS
  • RABWASH420X Wash Buffer (Item B)FDS

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Warning

Mentions de danger

H290

Conseils de prudence

P234 - P390

Classification des risques

Met. Corr. 1

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

FGF2 plays a key role in embryonic cerebrospinal fluid trophic properties over chick embryo neuroepithelial stem cells.
Martin C
Developmental Biology, 297(2), 402-416 (2006)
Jharna R Das et al.
PloS one, 11(4), e0153837-e0153837 (2016-04-21)
Renal endothelial cells (REc) are the first target of HIV-1 in the kidney. The integrity of REc is maintained at least partially by heparin binding growth factors that bind to heparan sulfate proteoglycans located on their cell surface. However, previous
Xiaohua Liu et al.
Psychiatry research, 218(1-2), 54-60 (2014-05-06)
Multiple neurotrophic and/or growth factors, recently nominated as "angioneurins", play the key roles in mood modulation and neuroplasticity, and their dysfunction might be involved in the pathophysiology and treatment of mood disorders. We examined serum levels of vascular endothelial growth
Sandeep Kumar Vishwakarma et al.
Materials science & engineering. C, Materials for biological applications, 102, 34-44 (2019-05-31)
Spinal cord injury (SCI) is one of the most precarious conditions which have been one of the major reasons for continuous increasing mortality rate of SCI patients. Currently, there is no effective treatment modality for SCI patients posing major threat
Yang Hu et al.
Molecular medicine reports, 13(1), 49-58 (2015-11-18)
Recent studies regarding regenerative medicine have focused on bone marrow mesenchymal stem cells (BMSCs), which have the potential to undergo neural differentiation, and may be transfected with specific genes. BMSCs can differentiate into neuron‑like cells in certain neurotropic circumstances in
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