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P4682

Sigma-Aldrich

Phénol solution

BioReagent, Saturated with 0.01 M citrate buffer, pH 4.3 ± 0.2, for molecular biology

Synonyme(s) :

nucleic acid extraction

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About This Item

Formule empirique (notation de Hill):
C6H6O
Numéro CAS:
108-95-2
Poids moléculaire :
94.11
Numéro Beilstein :
6428463
Numéro MDL:
MFCD00002143
Code UNSPSC :
12352104
ID de substance PubChem :
57654523
Nomenclature NACRES :
NA.52

Qualité

for molecular biology

Niveau de qualité

200

Gamme de produits

BioReagent

Forme

liquid

Technique(s)

RNA purification: suitable

pH

4.1-4.5 (25 °C)

Température de stockage

2-8°C

Chaîne SMILES 

Oc1ccccc1

InChI

1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H

Clé InChI

ISWSIDIOOBJBQZ-UHFFFAOYSA-N

Informations sur le gène

human ... GABRA1(2554)

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Description générale

Phenol solution is a clear liquid saturated with citrate buffer. Phenols are organic compounds that feature a hydroxyl group directly bonded to an aromatic carbocyclic ring. The common name for monohydroxybenzene is phenol.

Application

Phenol solution has been used:

  • for the extraction of RNA by standard phenol-chloroform protocols
  • in cell wall monosaccharide analysis to treat epidermal strips from the abaxial side of Commelina communisin leaves
  • as a component of lysis-denaturation buffer for RNA isolation

Actions biochimiques/physiologiques

Phenol solution can be used for a wide variety of molecular biology applications, including nucleic acid extractions using standard phenol-chloroform protocols. This organic solvent also serves as a potential protein denaturant. Phenol-chloroform extraction is a broadly used method for the extraction of nucleic acids. Despite phenol′s incomplete inhibition of RNase activity, it possesses characteristics that make it useful for isolating nucleic acids when combined with chloroform and alcohol.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Organes cibles

Nervous system,Kidney,Liver,Skin

Code de la classe de stockage

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Sagit Shalel-Levanon et al.
Biotechnology and bioengineering, 92(2), 147-159 (2005-07-01)
Escherichia coli has several elaborate sensing mechanisms for response to the availability of oxygen and the presence of other electron acceptors. The adaptive responses are coordinated by a group of global regulators, which include the one-component Fnr protein, and the
Jie Gao et al.
Proceedings of the National Academy of Sciences of the United States of America, 116(33), 16454-16462 (2019-07-04)
The programmed release of apoptogenic proteins from mitochondria is a core event of apoptosis, although ancestral roles of this phenomenon are not known. In mammals, one such apoptogenic protein is Endonuclease G (EndoG), a conserved mitochondrial nuclease that fragments the
Louise Jones et al.
Proceedings of the National Academy of Sciences of the United States of America, 100(20), 11783-11788 (2003-09-18)
Stomatal guard cells play a key role in the ability of plants to survive on dry land, because their movements regulate the exchange of gases and water vapor between the external environment and the interior of the plant. The walls
Thitiporn Anunthawan et al.
Biochimica et biophysica acta, 1848(6), 1352-1358 (2015-03-15)
We investigated the mechanisms of two tryptophan-rich antibacterial peptides (KT2 and RT2) obtained in a previous optimization screen for increased killing of both Gram-negative and Gram-positive bacteria pathogens. At their minimal inhibitory concentrations (MICs), these peptides completely killed cells of
Rita A Busuttil et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 20(10), 2761-2772 (2014-03-25)
Gene-expression profiling has revolutionized the way we think about cancer and confers the ability to observe the synchronous expression of thousands of genes. The use of putative genome-level expression profiles has allowed biologists to observe the complex interactions of genes
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