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P3803

Sigma-Aldrich

Phenol:Chloroform:Isoamyl Alcohol 25:24:1, Saturated with 10mM Tris, pH 8.0, 1mM EDTA

for molecular biology

Synonyme(s) :

DNA extraction

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About This Item

Code UNSPSC :
12190000
Nomenclature NACRES :
NA.52

Qualité

for molecular biology

Niveau de qualité

200

Pureté

≥99.0%

Forme

liquid

pH

6.5-6.9(pH of phenolic phase)

Application(s)

agriculture

Température de stockage

2-8°C

Application

Removal of proteins from nucleic acids can be achieved by extraction with phenol:chloroform: isoamyl alcohol solutions. For crude mixtures of nucleic acids, digestion with a broad range proteolytic enzyme prior to the phenol:chloroform extraction may be beneficial. Although proteins are efficiently denatured by phenol, RNase activity is not completely inhibited. Therefore, a small amount of isoamyl alcohol is added to further ensure the deactivation of RNase activity. Please see Product Information Sheet for the protocol for removal of proteins from nucleic acids.

Avertissement

If phenol solutions come in contact with skin, burns will occur quickly. It is, therefore, required that the user wear appropriate laboratory clothing including gloves and protective eyewear. In case of direct skin contact, rinse extensively with water and seek medical attention if necessary.

Autres remarques

This product has a shelf life of two years.

Produits recommandés

For DNA extraction, the pH of the phenol phase can be adjusted to 8.0 by equilibration with tris buffer. Sigma offers product P2069 specifically for DNA extractions. This product includes equilibration buffer for the convenience of adjusting the pH.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

08168

Timestrip Plus™ 8 °C

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3

Organes cibles

Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin

Code de la classe de stockage

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Philippe Lefrançois et al.
BMC genomics, 10, 37-37 (2009-01-23)
Short-read high-throughput DNA sequencing technologies provide new tools to answer biological questions. However, high cost and low throughput limit their widespread use, particularly in organisms with smaller genomes such as S. cerevisiae. Although ChIP-Seq in mammalian cell lines is replacing
Tzemach Aouizerat et al.
Bio-protocol, 10(1), e3473-e3473 (2020-01-05)
Ancient fermented food has been studied mainly based on residue analysis and recipes and reconstruction attempts were performed using modern domesticated yeast. Furthermore, microorganisms which participated in fermentation were studied using ancient-DNA techniques. In a recent paper, we presented a
Jeong-Kui Ku et al.
Annals of translational medicine, 8(6), 314-314 (2020-05-02)
The manufacturing of the demineralized dentin matrix (DDM) has been proven to extensively reduce the presence of human hepatitis B viral DNA (HBV DNA). This study measured and compared HBV DNA in fresh dentin to that in gamma radiation (GR)-sterilized
Stephan C A Wens et al.
Circulation. Cardiovascular genetics, 9(1), 6-13 (2016-01-21)
Elevated plasma cardiac troponin T (cTnT) levels in patients with neuromuscular disorders may erroneously lead to the diagnosis of acute myocardial infarction or myocardial injury. In 122 patients with Pompe disease, the relationship between cTnT, cardiac troponin I, creatine kinase
Ying Cheng et al.
Human molecular genetics, 27(17), 2955-2964 (2018-05-24)
Autism spectrum disorders (ASDs) include a group of syndromes characterized by impaired language, social and communication skills, in addition to restrictive behaviors or stereotypes. However, with a prevalence of 1.5% in developed countries and high comorbidity rates, no clear underlying
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