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P4557

Sigma-Aldrich

Phénol solution

Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, for molecular biology

Synonyme(s) :

Tris-equilabrated Phenol

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About This Item

Formule empirique (notation de Hill):
C6H6O
Numéro CAS:
108-95-2
Poids moléculaire :
94.11
Numéro Beilstein :
6428463
Numéro CE :
203-632-7
Numéro MDL:
MFCD00002143
Code UNSPSC :
12352104
ID de substance PubChem :
57654529
Nomenclature NACRES :
NA.52

Qualité

for molecular biology

Niveau de qualité

200

Gamme de produits

BioReagent

Pureté

≥99.0% (GC)

Forme

liquid

pH

7.7-8.1 (25 °C)

Température de stockage

2-8°C

Chaîne SMILES 

Oc1ccccc1

InChI

1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H

Clé InChI

ISWSIDIOOBJBQZ-UHFFFAOYSA-N

Informations sur le gène

human ... GABRA1(2554)

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Description générale

A tris-equilibrated phenol solution is a clear liquid capped with a layer of Tris buffer. Phenol solutions can be used for a wide variety of molecular biology applications. Organic compounds like phenols have an aromatic carbocyclic ring with a hydroxyl group attached directly to it. The common name for monohydroxybenzene is phenol.

Application

Phenol solution has been used:

  • as a component of the digestion reaction mix to prepare RNase-free plasmids for in vitro translation in a rabbit reticulocyte lysate
  • for extraction of DNA from Arabidopsis thaliana Rosette leaves
  • in the extraction of DNA from blood on preservation paper by standard phenol-chloroform protocol
  • as a component of extraction buffer to extract RNA from fruit pulp
  • for the extraction of protein from roots
  • for the isolation of fecal DNA
  • for the extraction of caged RNA

Actions biochimiques/physiologiques

The phenol-chloroform extraction method is widely employed to extract nucleic acids. Even though phenol does not completely inhibit RNase activity, its unique characteristic makes it beneficial for the isolation of nucleic acids when used in combination with chloroform and alcohol.

Composants

  • 1 bottle Tris-equilabrated Phenol (product number P4557)
  • Equilibration Buffer (product B5658)

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Organes cibles

Nervous system,Kidney,Liver,Skin

Code de la classe de stockage

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Michael B Powner et al.
Nature protocols, 7(6), 1086-1096 (2012-05-12)
The mouse retinal vasculature provides a powerful model system for studying development and pathologies of the vasculature. Because it forms as a two-dimensional flat plexus, it is easily imaged in its entirety in whole-mount retinal preparations. In order to study
Debby Bogaert et al.
PloS one, 6(2), e17035-e17035 (2011-03-10)
The nasopharynx is the ecological niche for many commensal bacteria and for potential respiratory or invasive pathogens like Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis. Disturbance of a balanced nasopharyngeal (NP) microbiome might be involved in the onset of symptomatic
Fecal Microbiome and Food Allergy in Pediatric Atopic Dermatitis: A Cross-Sectional Pilot Study
Fieten KB, et al.
International Archives of Allergy and Immunology, 175(1-2), 77-84 (2018)
Tim J Bull et al.
Journal of clinical microbiology, 41(7), 2915-2923 (2003-07-05)
Mycobacterium avium subsp. paratuberculosis is a robust and phenotypically versatile pathogen which causes chronic inflammation of the intestine in many species, including primates. M. avium subsp. paratuberculosis infection is widespread in domestic livestock and is present in retail pasteurized cows'
Thitiporn Anunthawan et al.
Biochimica et biophysica acta, 1848(6), 1352-1358 (2015-03-15)
We investigated the mechanisms of two tryptophan-rich antibacterial peptides (KT2 and RT2) obtained in a previous optimization screen for increased killing of both Gram-negative and Gram-positive bacteria pathogens. At their minimal inhibitory concentrations (MICs), these peptides completely killed cells of
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