GE28-9031-32

Protein A High Performance Spintrap^™

Cytiva 28-9031-32, pack of 16 columns

• Code UNSPSC : 41106500
• Nomenclature NACRES : NA.25

Propriétés

• ligand: native protein A
• Durée de conservation: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
• Caractéristiques: wetted part: no
• Conditionnement: pack of 16 columns
• Fabricant/nom de marque: Cytiva 28-9031-32
• Conditions de stockage: (20% Ethanol)
• Matrice: highly cross-linked 6% agarose
• Taille moy. des particules: 34 μm
• cleaning: 2-10(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
• working range: 3-9
• Capacité: >1 mg binding capacity(human IgG well)
• Température de stockage: 2-8°C

Description

Description générale

protein A HP SpinTrap^™ are designed for the enrichment of proteins of interest, immunoprecipitation and the purification of monoclonal and polyclonal antibodies from cell lysates and biological fluids. Contain protein A Sepharose^™ HP.

Application

Protein A Sepharose^™ HP has high affinity for the Fc region of IgG from a variety of species. The two options in the Protein enrichment protocol allow the attached antigen (Protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.

There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.

Caractéristiques et avantages

• For simple, small-scale antibody purification or enrichment of specific Proteins in prepacked, single-use spin columns that are ready to use.
• Classic and cross-link protocols provide flexibility.
• Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
• Fast binding kinetics and high capacity provide high yield.
• Prepacked with Protein A Sepharose^™ High Performance for coupling of antibodies of IgG subclasses.
• Easy scale-up with HiTrap^® Protein A HP prepacked columns.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

SpinTrap is a trademark of Cytiva

Informations sur la sécurité

• Pictogrammes: GHS02
• Mention d'avertissement: Warning
• Mentions de danger: H226
• Conseils de prudence: P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501
• Code de la classe de stockage: 3 - Flammable liquids