Accéder au contenu
MilliporeSigma
Toutes les photos(1)

Key Documents

GE28-4083-47

Ab Spintrap

Cytiva 28-4083-47

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.25

ligand

recombinant protein G lacking albumin-binding region

Conditionnement

pkg of 50 × 100 μL

Fabricant/nom de marque

Cytiva 28-4083-47

Conditions de stockage

(20% Ethanol)

Volume du lit

100 μL

Matrice

highly cross-linked 6% agarose

Taille moy. des particules

34 μm

cleaning

2-9

working range

3-9

Capacité

>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
>1 mg binding capacity(IgG/column)

Température de stockage

2-8°C

Description générale

Ab SpinTrap are prepacked, single-use spin columns for rapid purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants. Designed for small-scale purification of multiple samples in parallel.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Application

Ab SpinTrap is designed for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants. In addition, a protocol for immunoprecipitation is included.


The spin columns are also suitable for use in antibody screening experiments.


Ab SpinTrap columns are used together with a standard microcentrifuge and one purification run is performed in less than 20 min.


Ab SpinTrap contains Protein G Sepharose High Performance, which has high Protein binding capacity and is compatible with all commonly buffers used in antibody purification with immobilized Protein G media.


For optimal performance in antibody purification, use Ab SpinTrap together with buffers prepared using Ab Buffer Kit.

Caractéristiques et avantages

  • Rapid and convenient small-scale purification of IgG.
  • Protein G Sepharose High Performance in prepacked spin columns.
  • No filtration or centrifugation of different crude samples necessary for antibody purification.
  • Classic and crosslink protocols for Protein enrichment (immunoprecipitation).
  • Elution conditions formatted for both LC-MS and electrophoresis analysis.
  • Elution conditions formatted for both LC-MS and electrophoresis analysis.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva

Pictogrammes

Flame

Mention d'avertissement

Warning

Mentions de danger

Code de la classe de stockage

3 - Flammable liquids


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Les clients ont également consulté

Articles

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

Protocoles

This page shows how to separate IgG antibodies by affinity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from GE Healthcare.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique