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G7663

Sigma-Aldrich

Gelatin blocking buffer

for Western blotting, powder blend

Synonyme(s) :

blocking buffer

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About This Item

Code UNSPSC :
41105300
Nomenclature NACRES :
NA.25

Qualité

for molecular biology

Niveau de qualité

100

Forme

powder blend

Température de stockage

room temp

Description générale

Gelatin Blocking Buffer is a standard reagent in Western blotting procedures. It is used to block non-specific binding on the membrane, when using nylon or nitrocellulose (but not PVDF).

Application

Suitable for use as a non-specific blocking agent for Western blots.

Reconstitution

Dissolve entire contents of the bottle in a final volume of 1L molecular biology grade water. The resulting solution will be faint yellow in color and does not require filtering or heating before use.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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A Fortin et al.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 72(5-6), 239-243 (1994-05-01)
The major advantages of the horseradish peroxidase chemiluminescence (HRP-CL) immunodetection method in Western blot analysis are its high sensitivity, nonradioactive detection, economy of the primary antibody, and speed of detecting the signal. However, we observed a strong and reproducible signal
K A Brogden et al.
Infection and immunity, 67(8), 4256-4259 (1999-07-23)
Affinity-purified rabbit polyclonal (PAB96-1) and mouse monoclonal (1G9-1C2) antibodies to synthetic H-DDDDDDD-OH, an antimicrobial anionic peptide (AP) originally isolated from ovine pulmonary surfactant, were prepared and used to assess the concentrations of AP-like molecules in human respiratory tract samples. In
A sensitive cell-based assay for the detection of residual infectious West Nile virus.
Koldijk, M.H., et al.
Vaccine, 25, 39-39 (2007)
Raul Bettencourt et al.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 152(2), 278-289 (2008-12-02)
The interaction between microorganisms and host defense mechanisms is a decisive factor for the survival of marine bivalves. They rely on cell-mediated and humoral reactions to overcome the pathogens that naturally occur in the marine environment. In order to understand
Iram Liaqat et al.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 43(3), 969-980 (2012-07-01)
Enterotoxigenic Escherichia coli (ETEC) strains are leading causes of childhood diarrhea in developing countries. Adhesion is the first step in pathogenesis of ETEC infections and ETEC pili designated colonization factor antigens (CFAs) are believed to be important in the biofim
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Protocoles

Gelatin Blocking Buffer for Direct Detection Southern or Western Blotting

In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.

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