G5160
β-Galactosidase from Aspergillus oryzae
≥8.0 units/mg solid
Synonyme(s) :
β-D-Galactoside galactohydrolase, Lactase from Aspergillus oryzae
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About This Item
Produits recommandés
Forme
solid
Niveau de qualité
Activité spécifique
≥8.0 units/mg solid
Contient
dextrin as extender
Température de stockage
−20°C
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Catégories apparentées
Description générale
β-Galactosidase is a member of the glycoside hydrolase clan A (GH-A) family. It is widely present in microorganisms, which includes both prokaryotes and eukaryotes.
Application
β-Galactosidase from Aspergillus oryzae has been used:
- in in vitro nuclear magnet resonance (NMR)
- as a standard in the β-galactosidase assay to determine the concentration of cell-associated β-galactosidase
- to modify sugar residues on the surface of the mutant slime cells to study the adhesive properties
- for enzymatic hydrolysis of lactose
- for reversed-phase (RP) adsorption
- in the hydrolysis of whey lactose
Actions biochimiques/physiologiques
β-Galactosidase acts on the terminal β-D-galactosyl moieties of disaccharides, glycoconjugates, and polysaccharides. It is useful in mediating transglycosylation reactions such as the synthesis of galactooligosaccharides (GOS). β-Galactosidases might be used for glycobiological and biotechnological applications.
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
Définition de l'unité
1 U hydrolysiert 1.0 μmol o-Nitrophenyl β-D-Galactosid zu o-Nitrophenol und D-Galactose pro Minute beim angegebenen pH- und Temperatur-Wert, falls nicht anders erwähnt.
Inhibiteur
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
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Hydrolysis of whey lactose by immobilized β-Galactosidase
Marcela Panaro Mariotti; Hideko Yamanaka; Angela Regina Araujo; Henrique Celso Trevisan
Brazilian Archives of Biology and Technology, 51 (2008)
Modular process for the flexible synthesis of magnetic beads-Process and product validation
Birgit Hickstein1, and Urs Alexander Peuker
Journal of Applied Polymer Science, 112, 2366-2373 (2009)
Jian-Xin Yu et al.
NMR in biomedicine, 21(7), 704-712 (2008-02-22)
Gene therapy has emerged as a promising strategy for treatment of various diseases. However, widespread implementation is hampered by difficulties in assessing the success of transfection in the target tissue and the longevity of gene expression. Thus, there is increasing
A Y Dunina-Barkovskaya et al.
The Journal of membrane biology, 198(2), 77-87 (2004-05-13)
Low gadolinium concentrations induce rapid gigaseal formation and cell adhesion to glass and plastic (polystyrene) substrates in the slime mutant of Neurospora crassa. Cellular adhesion is independent of an integrin-mediated mechanism, because pretreatment with the oligopeptide ARG-GLY-ASP-SER (RGDS) did not
Daniel Muñoz-Espín et al.
EMBO molecular medicine, 10(9) (2018-07-18)
Senescent cells accumulate in multiple aging-associated diseases, and eliminating these cells has recently emerged as a promising therapeutic approach. Here, we take advantage of the high lysosomal β-galactosidase activity of senescent cells to design a drug delivery system based on
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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