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D9944

Sigma-Aldrich

Monoclonal Anti-DYNLT1 antibody produced in mouse

~1.0 mg/mL, clone T1, purified immunoglobulin, buffered aqueous solution

Synonyme(s) :

Anti-CW-1, Anti-Dynein, light chain, Tctex-type 1, Anti-TCTEL1, Anti-Tctex-1

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

T1, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~12.5 kDa

Espèces réactives

monkey, mouse, bovine, human, rat

Concentration

~1.0 mg/mL

Technique(s)

immunocytochemistry: suitable
western blot: 8-16 μg/mL using NRK total cell lysate

Isotype

IgG1

Numéro d'accès UniProt

P63172

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... DYNLT1(6993)
mouse ... Dynlt1(21648)
rat ... Dynlt1(83462)

Immunogène

recombinant dynein 1 light chain-myosin basic protein fusion protein.

Application

Monoclonal Anti-DYNLT1 antibody produced in mouse is suitable for immunocytochemistry and immunoblotting at a working concentration of 8-16μg/mL using NRK total cell lysate.

Actions biochimiques/physiologiques

Cytoplasmic dynein motor complex is responsible for minus-end, microtubule-based motile processes. It is thus involved in a wide range of eukaryotic cellular processes including trafficking of membranal vesicles, viruses and other intracellular particles. DYNLT1 is a light chain subunit of the complex that is involved in cargo binding. It binds specifically to the cytoplasmic tail of rhodopsin and links rhodopsin-bearing vesicles to the dynein motor complex for transport. The cargo is released by the disassembly of dynein complex upon phosphorylation of DYNLT1 on Ser82 residue. This process is critical for the apical delivery of membrane cargoes. It also mediates neurite extension by modulating actin dynamics and also Rac1 activity. The abundance and expression pattern of DYNLT1 in neuronal tissues may also contribute to the invasion capabilities of neurotropic viruses like poliovirus.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

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N Hirokawa
Science (New York, N.Y.), 279(5350), 519-526 (1998-02-07)
Cells transport and sort proteins and lipids, after their synthesis, to various destinations at appropriate velocities in membranous organelles and protein complexes. Intracellular transport is thus fundamental to cellular morphogenesis and functioning. Microtubules serve as a rail on which motor
Steffen Mueller et al.
The Journal of biological chemistry, 277(10), 7897-7904 (2001-12-26)
The cellular receptor for poliovirus CD155 (or PVR) is the founding member of a new class of membrane-associated immunoglobulin-like proteins, which include the mouse tumor-associated antigen E4 (Tage4) and three proteins termed "nectins." Using a yeast two-hybrid screen we have
A W Tai et al.
The Journal of cell biology, 153(7), 1499-1509 (2001-06-27)
Despite the existence of multiple subunit isoforms for the microtubule motor cytoplasmic dynein, it has not yet been directly shown that dynein complexes with different compositions exhibit different properties. The 14-kD dynein light chain Tctex-1, but not its homologue RP3
Ting-Yu Yeh et al.
Traffic (Copenhagen, Denmark), 7(11), 1495-1502 (2006-09-08)
Post-Golgi to apical surface delivery in polarized epithelial cells requires the cytoplasmic dynein motor complex. However, the nature of dynein-cargo interactions and their underlying regulation are largely unknown. Previous studies have shown that the apical surface targeting of rhodopsin requires
Jacques Brocard et al.
The European journal of neuroscience, 46(11), 2754-2767 (2017-11-03)
MAP6 proteins were first described as microtubule-stabilizing agents, whose properties were thought to be essential for neuronal development and maintenance of complex neuronal networks. However, deletion of all MAP6 isoforms in MAP6 KO mice does not lead to dramatic morphological
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