C7698

Cycloheximide

from microbial, ≥94% (TLC)

• Synonyme(s) : 3-[2-(3,5-Diméthyl-2-oxocyclohexyl)-2-hydroxyéthyl]glutarimide, Actidione, Naramycine A
• Formule empirique (notation de Hill): C₁₅H₂₃NO₄
• Numéro CAS: 66-81-9
• Poids moléculaire : 281.35
• Numéro Beilstein : 88868
• Numéro CE : 200-636-0
• Numéro MDL: MFCD00082346
• Code UNSPSC : 12352111
• ID de substance PubChem : 24892981
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: microbial
• Niveau de qualité: 200
• Pureté: ≥94% (TLC)
• Forme: powder
• Conditions de stockage: (Tightly closed. Dry. Keep in a well-ventilated place. Keep locked up or in an area accessible ; only to qualified or authorized persons.)
• Couleur: white to off-white
• Solubilité: ethanol: soluble, clear to hazy
• Spectre d'activité de l'antibiotique: fungi; yeast
• Mode d’action: protein synthesis | interferes
• Température de stockage: 2-8°C
• Chaîne SMILES: [H][C@]1(C[C@@H](C)C[C@H](C)C1=O)[C@H](O)CC2CC(=O)NC(=O)C2
• InChI: 1S/C15H23NO4/c1-8-3-9(2)15(20)11(4-8)12(17)5-10-6-13(18)16-14(19)7-10/h8-12,17H,3-7H2,1-2H3,(H,16,18,19)/t8-,9-,11-,12+/m0/s1
• Clé InChI: YPHMISFOHDHNIV-FSZOTQKASA-N
• Informations sur le gène: human ... FKBP1A(2280) , PIN1(5300)

Description

Description générale

Cycloheximide, also known as Actidione, is a glutarimide antibiotic commonly derived from the bacterium Streptomyces griseus. It acts as a potent inhibitor of protein biosynthesis in eukaryotic cells by disrupting the translocation step during translation, effectively blocking translational elongation. In microbiology, it plays a crucial role as a selection agent for resistant strains of yeast and fungi, proving invaluable in controlled experimental environments.

In cell biology and biochemical research, Cycloheximide showcases a dual nature concerning Apoptosis Induction, inducing or inhibiting apoptosis depending on the cell type. Its rapid and reversible effects make it an ideal choice for studying cellular processes and determining protein half-life. Cycloheximide also finds extensive applications in biomedical research, where it inhibits protein synthesis in eukaryotic cells studied in vitro (outside of organisms) and its effects are rapidly reversed by simply removing it. This makes Cycloheximide a go-to choice for exploring cell biology, biomedical and biochemical research, offering precise control and versatility in experiments.

Application

• La cycloheximide a été utilisée comme inhibiteur de la synthèse des protéines dans certaines souches de levure dans le cadre d′expériences de chasse à la cycloheximide.
• Elle a été employée pour inhiber la traduction dans les cellules de mammifères
• Elle a été employée pour inhiber la croissance fongique.

Actions biochimiques/physiologiques

Mode of Action: Translation inhibition in eukaryotes resulting in cell growth arrest and cell death. CHX is widely used for the selection of CHX-resistant strains of yeast and fungi, controlled inhibition of protein synthesis for detection of short-lived proteins and super-induction of protein expression, and apoptosis induction or facilitation of apoptosis induction by death receptors.

Activity Spectrum: Active against yeast and fungi like Candida, Aspergillus, Saccharomyces, Penicillium

Caractéristiques et avantages

• High-quality antibiotic suitable for multiple research applications
• Commonly used in Cell Biology and Biochemical applications

Autres remarques

Conserver le récipient bien fermé et dans un endroit sec et bien ventilé. Conserver dans un endroit sec.

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Informations sur la sécurité

• Pictogrammes: GHS06,GHS08,GHS09
• Mention d'avertissement: Danger
• Mentions de danger: H300 - H341 - H360D - H411
• Conseils de prudence: P202 - P264 - P273 - P280 - P301 + P310 - P391
• Classification des risques: Acute Tox. 2 Oral - Aquatic Chronic 2 - Muta. 2 - Repr. 1B
• Code de la classe de stockage: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges