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A1935

Sigma-Aldrich

Photobiotin acetate salt

For labeling DNA probes for hybridizations

Synonyme(s) :

Biotin {3-[3-(4-azido-2-nitroanilino)-N-methylpropylamino]propylamide} acetate salt, N-(4-Azido-2-nitrophenyl)-N′-(3-biotinylaminopropyl)-N′-methyl-1,3-propanediamine acetate salt

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About This Item

Formule empirique (notation de Hill):
C23H35N9O4S · C2H4O2
Numéro CAS:
Poids moléculaire :
593.70
Numéro MDL:
Code UNSPSC :
12352200
ID de substance PubChem :
Nomenclature NACRES :
NA.52

Qualité

for molecular biology

Niveau de qualité

Pureté

≥95%

Forme

film or powder

Solubilité

H2O: 10 mg/mL (Stable for at least 5 months if kept frozen and protected from light.)

Température de stockage

−20°C

Chaîne SMILES 

CC(O)=O.CN(CCCNC(=O)CCCC[C@@H]1SCC2NC(=O)NC12)CCCNc3ccc(cc3[N+]([O-])=O)N=[N+]=[N-]

InChI

1S/C23H35N9O4S.C2H4O2/c1-31(12-4-10-25-17-9-8-16(29-30-24)14-19(17)32(35)36)13-5-11-26-21(33)7-3-2-6-20-22-18(15-37-20)27-23(34)28-22;1-2(3)4/h8-9,14,18,20,22,25H,2-7,10-13,15H2,1H3,(H,26,33)(H2,27,28,34);1H3,(H,3,4)/t18-,20-,22-;/m0./s1

Clé InChI

FFBLNTOMOSLSQM-AYEYRVMASA-N

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Description générale

Photobiotin acetate is made up of biotin, which is bound with the help of a charged linker arm to a photoreactive arylazide group.

Application

Photobiotin acetate salt has been used to label DNA probes to perform DNA−DNA hybridization to determine genomic relatedness.

Actions biochimiques/physiologiques

Photobiotin acetate is capable of labeling single stranded DNA and linear or supercoiled double-stranded DNA.
Photobiotin is a photo-activatable analog of biotin used primarily for labeling DNA, RNA and proteins. Probes prepared using photobiotin can detect target DNA as little as 0.5 pg.

Attention

Light sensitive.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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H Zreiqat et al.
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