D9905
Denhardt′s Solution
for Northern and Southern blotting, lyophilized powder
Synonyme(s) :
Denhardt′s Solution For Blotting Technique
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About This Item
Code UNSPSC :
12161700
Nomenclature NACRES :
NA.25
Produits recommandés
Qualité
for molecular biology
Forme
lyophilized powder
Technique(s)
Southern blotting: suitable
Activité étrangère
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
Température de stockage
2-8°C
Description générale
Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.
Application
Denhardt′s Solution has been used:
- as a component in hybridization buffer for the hybridization of brain sections
- in in-situ hybridization peroxidase-(antiperoxidase method-whole mounts) (ISHPAP-WM) process
- in the preparation of hybridization mixture
Suitable as a blocking Buffer for Southern and Northern blot membranes.
Actions biochimiques/physiologiques
Denhardt′s solution and gelatin-chrome alum can be used to treat chromosomes for G-banding with trypsin after in situ hybridization.
Composants
Each vial of Denhardt′s powder contains 50mg Bovine Serum Albumin (BSA), 50mg Ficoll, and 50mg Polyvinylpyrrolidone (PVP).
Notes préparatoires
To make a 50x concentrate, dissolve entire contents of vial in 5 mL molecular biology grade water. Store at -20°C for up to two years.
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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