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04942086001

Roche

Dispase® I (neutral protease, grade I)

lyophilized, ≥10 units/mg protein (20 U/vial (37 °C, casein as substrate, pH 7.5)), optimum pH 6.0-8.5

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About This Item

Numéro de classification (Commission des enzymes):
3.4.24.4 (BRENDA, IUBMB)
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.21

Source biologique

Bacillus polymyxa

Niveau de qualité

100

Forme

lyophilized

Activité spécifique

≥10 units/mg protein (20 U/vial (37 °C, casein as substrate, pH 7.5))

Conditionnement

pkg of 10 × ~2 mg

Fabricant/nom de marque

Roche

Concentration

0.6-2.4 U/mL

Technique(s)

tissue processing: suitable

Couleur

white

pH optimal

6.0-8.5

Solubilité

water: soluble

Application(s)

life science and biopharma
sample preparation

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

Dispase is a neutral, rapid-acting, gentle protease that separates intact epidermis from dermis. It also separates cultured intact epithelial cells from the substratum. It cleaves the basement membrane zone and leaves the epithelial cells intact.

Spécificité

Dispase® is a non-specific metalloprotease.

Application

Dispase® is used for the preparation of cells from a wide variety of different tissues and organs. The enzyme has proven to be a rapid, effective, yet gentle agent for the disruption of extracellular matrix of tissues for releasing single cells for cell culture (primary cell culture) or harvesting cells already in culture to transfer to new substrate (secondary culture). Furthermore, it is used to prevent unwanted clumping of cells cultured in suspension.

Caractéristiques et avantages

  • Rapid, effective, yet gentle agent that liberates intact cells
  • Maintains cell membrane integrity
  • Non-mammalian source - free of mycoplasma and animal virus contamination
  • Extremely stable to influences of temperature, pH, and interference by serum components
  • Easily inactivated by chelating agents or by dilution
  • Delivers higher activity and convenience

Contents
10 vials with approximately 2mg each (= 20U/vial).

Caractéristiques

Enzyme activity: =10U/mg (+37°C, casein as substrate, pH 7.5)
Inhibitors: EDTA, EGTA, Hg2+, other heavy metals. Dispase is not inhibited by serum.

Définition de l'unité

One unit is the enzyme activity which liberates Folin-positive amino acids and peptides from casein corresponding to 1 μmol (181 μg) tyrosine in 1 minute under assay conditions (pH 7.5, +37 °C).
A practical comparison of RAS units of Dispase® with those cited in the Japanese literature (where concentrations of 1,000 to 2,000 Dispase® units /ml are not uncommon) suggests one Roche Applied Science unit of Dispase® I equals approximately 416 Japanese units of Dispase®.
One unit of Roche Applied Science Dispase® equals 181 protease units (PU) measured as release of amino acids equivalent to 1 μg tyrosine per min and ml at pH 7.5 and +37 °C.

Forme physique

white lyophilizate, before lyophilization filtered through 0.2 μm pore size membrane

Notes préparatoires

Activator: Optimal Ca2+ concentration is 2 mM. The enzyme preparations contain enough Ca2+ for optimal activity.
Working concentration: 0.6 to 2.4 U/ml
Working solution: Preparation of stock and working solutions:
To produce a 10 mg/ml stock solution, dissolve the lyophilized Dispase® I enzyme in HEPES-buffered saline (50 mM HEPES/KOH pH 7.4, 150 mM NaCl). To produce the working solution, dilute the above stock solution with the culture medium for the isolated cells, at a final concentration of 0.6 to 2.4 U/ml. Note that concentrations higher than 2.4 U/ml are not recommended. For best results, filter the working solution using a 0.22 μm filter membrane.
Storage conditions (working solution): -15 to -25 °C
The reconstituted stock solution is stable at 2 to 8 °C for 2 weeks. For storage up to 2 months, the stock solution should be frozen in aliquots. Avoid repeated freezing and thawing.
The working solution diluted with PBS is stable at 2 to 8 °C for 3 days.

Stockage et stabilité

Store at 2 to 8 °C. (Store dry!)

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations légales

Dispase is a registered trademark of Godo Shusei Co., Ltd.

Pictogrammes

Exclamation markHealth hazard
GHS07,GHS08

Mention d'avertissement

Danger

Mentions de danger

H315 - H319 - H334 - H335

Classification des risques

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Arvydas Maminishkis et al.
Journal of visualized experiments : JoVE, (45)(45), doi:10-doi:10 (2010-11-19)
We have developed a cell culture procedure that can produce large quantities of confluent monolayers of primary human fetal retinal pigment epithelium (hfRPE) cultures with morphological, physiological and genetic characteristics of native human RPE. These hfRPE cell cultures exhibit heavy
Katrin Hack et al.
PloS one, 7(5), e37097-e37097 (2012-05-19)
We have previously shown that peroxisome proliferator activating receptor ß/δ (PPAR β/δ is overexpressed in psoriasis. PPAR β/δ is not present in adult epidermis of mice. Targeted expression of PPAR β/δ and activation by a selective synthetic agonist is sufficient
Monika Haemmerle et al.
Diabetes, 62(7), 2509-2529 (2013-02-21)
Type 2 diabetes is associated with microvascular damage that causes frequent infections in the skin and chronic ulcers as a result of impaired wound healing. To trace the pathological changes, we performed a comprehensive analysis of lymphatic vessels in the
K S Stenn et al.
The Journal of investigative dermatology, 93(2), 287-290 (1989-08-01)
Dispase, a neutral protease isolated from culture filtrates of Bacillus polymyxa, has proven to be a rapid, effective, but gentle agent for separating intact epidermis from the dermis and intact epithelial sheets in culture from the substratum. In both cases

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