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Key Documents

03-189

Sigma-Aldrich

CBP (catalytic domain) human recombinant

CBP (catalytic domain) human recombinant protein expressed in E. Coli.

Synonyme(s) :

Human CBP (catalytic domain) protein, Human recombinant CBP, Upstate® human recombinant CBP (catalytic domain)

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.54

Source biologique

human

Niveau de qualité

100

Pureté

>65% (SDS-PAGE)

Poids mol.

~48 kDa

Fabricant/nom de marque

Upstate®

Technique(s)

enzyme immunoassay: suitable

Numéro d'accès UniProt

Q92793

Description générale

Although originally termed histone acetyltransferases (HATs) for their lysine acetylation activity on histone N-terminal tails, p300 and its paralogue, CBP, have been shown to acetylate a variety of non-histone proteins including p53, DNA polymerase ß and nuclear import factors. p300/CBP acetylations play regulatory roles in transcription, DNA repair and replication, the cell cycle, p53 turnover, and nuclear import. In addition to functions which overlap with those of CBP, p300 has a number of unique functions, including a role in the induction of p21Waf1/Cip1 cell cycle inhibitor.

Source:
Catalytic domain (residues 1319-1710) of human CBP histone acetyltransferase

Spécificité

[Varies with lot. For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.] XXX 250 pmol/min/µg assayed as production of CoA-SH from AcCoA in the presence of a peptide comprising human p53 residues 368-386. CoA-SH is determined colorimetrically by reaction with DTNB (5,5′-Dithiobis (2-nitrobenzoic acid).

Application

Acetyltransferase assays
CBP (catalytic domain) human recombinant protein expressed in E. Coli.

Forme physique

Format: Purified
In liquid containing 50 mM Tris/HCl, pH 8.0, 0.1 mM EDTA, 10% glycerol. Frozen solution.

Stockage et stabilité

Stable for 12 months at -80°C from date of receipt. Avoid multiple freeze/thaw cycles.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

10 - Combustible liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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