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Key Documents

03-188

Sigma-Aldrich

p300 (catalytic domain) human recombinant

Human recombinant p300 (catalytic domain) expressed in E. Coli.

Synonyme(s) :

Human recombinant p300 (catalytic domain) expressed in E. Coli, Upstate® human recombinant p300 (catalytic domain), p300 (catalytic domain) expressed in E. Coli

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About This Item

Code UNSPSC :
12352200

Source biologique

Escherichia coli

Niveau de qualité

100

Pureté

>75% (SDS-PAGE)

Poids mol.

~52 kDa

Fabricant/nom de marque

Upstate®

Technique(s)

enzyme immunoassay: suitable

Description générale

Human recombinant p300 (catalytic domain) expressed in E. Coli
Although originally termed histone acetyltransferases (HATs) for their lysine acetylation activity on histone N-terminal tails, p300 and its paralogue, CBP, have been shown to acetylate a variety of non-histone proteins including p53, DNA polymerase ß and nuclear import factors. p300/CBP acetylations play regulatory roles in transcription, DNA repair and replication, the cell cycle, p53 turnover, and nuclear import. In addition to functions which overlap with those of CBP, p300 has a number of unique functions, including a role in the induction of p21Waf1/Cip1 cell cycle inhibitor.

Spécificité

>250 pmol/min/µg assayed as production of CoA-SH from AcCoA in the presence of a peptide comprising human p53 residues 368-386. CoA-SH is determined colorimetrically by reaction with DTNB (5,5′-Dithiobis (2-nitrobenzoic acid).

Application

Human recombinant p300 (catalytic domain) expressed in E. Coli.

Acetyltransferase assays
Enzyme Assays

Forme physique

Format: Purified
In liquid containing 50 mM Tris/HCl, pH 8.0, 0.1 mM EDTA, 10% glycerol. Frozen solution.

Stockage et stabilité

Stable for 12 months at -80ºC from date of receipt. Avoid multiple freeze/thaw cycles.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

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