760706

Sulfo-dibenzocyclooctyne-biotin conjugate

for Copper-free Click Chemistry

• Synonyme(s) : Sulfo-DBCO-biotin
• Formule empirique (notation de Hill): C₃₇H₅₀N₆O₇S₂
• Poids moléculaire : 754.96
• Code UNSPSC : 12352200
• ID de substance PubChem : 329766890
• Nomenclature NACRES : NA.22

Propriétés

• Forme: solid
• Capacité de réaction: reaction type: click chemistry
• Pertinence de la réaction: reagent type: cross-linking reagent
• Température de stockage: −20°C
• Chaîne SMILES: CC[NH+](CC)CC.[O-]S(=O)(=O)C(CNC(=O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@H]12)C(=O)NCCC(=O)N3Cc4ccccc4C#Cc5ccccc35
• InChI: 1S/C31H35N5O7S2.C6H15N/c37-27(12-6-5-11-25-29-23(19-44-25)34-31(40)35-29)33-17-26(45(41,42)43)30(39)32-16-15-28(38)36-18-22-9-2-1-7-20(22)13-14-21-8-3-4-10-24(21)36;1-4-7(5-2)6-3/h1-4,7-10,23,25-26,29H,5-6,11-12,15-19H2,(H,32,39)(H,33,37)(H2,34,35,40)(H,41,42,43);4-6H2,1-3H3/t23-,25-,26?,29-;/m0./s1
• Clé InChI: RACLEVCKGUBCTH-GESOYTMLSA-N

Description

Description générale

This sulfonated azadibenzocyclooctyne-biotin derivative is a highly water soluble and versatile biotinylation reagent for labeling azide containing molecules or compounds. Cyclooctynes are useful in strain-promoted copper-free azide-alkyne cycloaddition reactions.This dibenzocyclooctyne will react with azide-functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage. Subsequent labeled molecules can be purified using streptavidin or avidin affinity reagents.

Application

Automate your Biotin tagging with Synple Automated Synthesis Platform (SYNPLE-SC002)

Caractéristiques et avantages

More efficient labeling will occur at higher temperatures and with greater excess of biotin reagent. Typical reaction times are up to 4 hours, but longer incubation periods should be used if concentration is low or if low labeling is observed. Product is most efficient when first dissolved in a water miscible organic solvent and then diluted in aqueous reaction buffer when needed. Stock solutions in DMSO have been shown to be stable in the freezer for up to 3 months.

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H315,H319,H335
• Conseils de prudence: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable