G9295
Monoclonal Anti-GAPDH−Peroxidase antibody produced in mouse
clone GAPDH-71.1, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Anti-G3PD, Anti-G3PDH, Anti-Glyceraldehyde-3-phosphate Dehydrogenase
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About This Item
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biological source
mouse
Quality Level
conjugate
peroxidase conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
GAPDH-71.1, monoclonal
form
buffered aqueous solution
mol wt
antigen ~37 kDa
species reactivity
rabbit, canine, rat, hamster, monkey, mouse, turkey, bovine, mink, human, chicken
should not react with
prokaryotes
technique(s)
western blot: 1:35,000-1:70,000 using A431 total cell extract
isotype
IgM
UniProt accession no.
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... GAPDH(2597)
mouse ... Gapdh(14433)
rat ... Gapdh(24383)
General description
Monoclonal Anti-GAPDH (mouse IgM isotype) is derived from the hybridoma GAPDH-71.1 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with rabbit GAPDH.
The gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is mapped to human chromosome 12p13. The protein localizes in the cytoplasm but can be translocated to the nucleus depending on cellular conditions.
Specificity
The antibody does not crossreact with non-vertebrate and prokaryote GAPDH.
Immunogen
rabbit GAPDH.
Application
Monoclonal Anti-GAPDH-Peroxidase antibody produced in mouse has been used in
- immunoblotting
- enzyme linked immunosorbent assay (ELISA)
- immunocytochemistry
Biochem/physiol Actions
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a tetramer containing identical chains. It catalyzes the reversible oxidative phosphorylation of glyceraldehyde-phosphate, which is a critical energy-yielding step in carbohydrate metabolism. It binds to several proteins including actin, tubulin, amyloid precursor, polyglutamine peptides, DRPLA (dentatorubral-pallidoluysian atrophy), and huntingtin. Protein kinase Cι/λbinds and phosphorylates GAPDH. Phosphorylated GAPDH associates with cytoskeletal elements and controls microtubule dynamics in the early secretory pathway. Poly(ADP-ribose) polymerase-1 (PARP1) interacts with GAPDH and thereby mediates brain damage in the presence of oxidative/nitrosative stress. GAPDH forms a part of OCA-S, the multicomponent OCT1 (octamer-motif-binding factor) coactivator complex, which is involved in the S phase-dependent histone H2B transcription. This association is responsible for linking H2B transcriptional machinery to cell cycle regulation and to the cellular metabolic state. GAPDH is also a component of the functional GAIT (interferon-γ-activated inhibitor of translation) mRNP (messenger ribonucleoprotein). GAPDH expression is dysregulated during melanoma progression.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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