A3854

Anti-β-Actin−Peroxidase antibody, Mouse monoclonal

clone AC-15, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-β-Actin, Loading Control
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: peroxidase conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: AC-15, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 42 kDa
• species reactivity: sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig
• should not react with: Dictyostelium discoideum; Drosophila
• technique(s): western blot: 1:25,000-1:50,000 using cell extracts of human foreskin fibroblasts or chicken fibroblasts
• isotype: IgG1
• UniProt accession no.: P60709
• application(s): research pathology
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ACTB(60) ; mouse ... Actb(11461) ; rat ... Actb(81822)

Description

General description

Actin is one of the most conserved eukaryotic proteins and is expressed in mammals and birds as at least six isoforms. There are three α-actins (skeletal, cardiac, and smooth muscle), one β-actin (β-nonmuscle), and two γ-actins (γ-smooth muscle and γ-non muscle). Actin isoforms show >90% overall sequence homology, but only 50–60% homology in their NH2-terminal residues. ACTB (actin β) gene codes for β-actin. It is a cytoskeletal housekeeping protein. It is present in the cytoplasm. The ACTB gene is located on human chromosome 7p22.1.
Mouse monoclonal anti-β-actin-peroxidase antibody specifically localizes β-actin in a wide variety of tissues and species using immunoblotting (42kDa). The antibody cross-reacts with β-Actin expressed in cells of human, bovine, sheep, pig, rabbit, cat, dog, mouse, rat, guinea pig, chicken, carp, and Hirudo medicinalis (leech) tissues, but not in Dictyostelium discoideum amoebae or Drosophila.

In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used.

Mouse monoclonal anti-β-actin-peroxidase antibody specifically localizes β-actin in a wide variety of tissues and species using immunoblotting (42kDa). The antibody cross-reacts with β-Actin expressed in cells of human, bovine, sheep, pig, rabbit, cat, dog, mouse, rat, guinea pig, chicken, carp, and Hirudo medicinalis (leech) tissues, but not in Dictyostelium discoideum amoebae or Drosophila.

Immunogen

slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

Application

Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.

Monoclonal mouse anti-actin was used as a loading control for western blot analysis of rat liver protein lysates.

Monoclonal mouse anti-beta actin antibody is suitable as a loading control or primary antibody for western blot analysis. It has also been used in chemiluminescent detection.

Biochem/physiol Actions

The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. The six isoforms of actins are characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found practically in all cells. β-Actin (ACTB) modulates cell growth, migration and the G-actin pool. Mutations in ACTB leads to pleiotropic developmental disorder.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves