ZRB374
Anti-GAPDH Antibody, clone 10B13, ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
EC: 1.2.1.12, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit (recombinant)
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
10B13, monoclonal
recombinant monoclonal
description
10B13 Clone
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 36.05 kDa
observed mol wt ~36 kDa
species reactivity
human
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
immunofluorescence: suitable using 1:100
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using 1:100
western blot: suitable using 1:1,000
isotype
IgG
UniProt accession no.
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... GAPDH(2597)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Specificity
Clone 10B13 is a ZooMab rabbit recombinant monoclonal antibody that specifically detects human Glyceraldehyde-3-phosphate dehydrogenase and targets an epitope with in the C-terminal half.
Immunogen
GST/His-tagged recombinant fragment corresponding to 190 amino acids from the C-terminal half of human Glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
Application
Anti-GAPDH, clone 10B13 ZooMAb, Cat. No. ZRB374, is a a recombinant Rabbit Monoclonal Antibody that specifically targets Glyceraldehyde-3-phosphate dehydrogenase and ha been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Wetern Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GAPDH in HEK293 cell lysate.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected GAPDH in human testis tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GAPDH in human testis tissue sections.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected GAPDH in human testis tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GAPDH in human testis tissue sections.
Target description
Glyceraldehyde-3-phosphate dehydrogenase (UniProt: P04406; also known as EC: 1.2.1.12, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH) is encoded by the GAPDH (also known as GAPD, CDABP0047, OK/SW-cl.1) gene (Gene ID: 2597) in human. GAPDH is considered as a ′house-keeping′ enzyme that catalyzes the synthesis of 1,3-biphosphoglycerate. It is a homotetrameric enzyme with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities. Hence, it plays a role in both glycolysis and nuclear functions. It translocates to the nucleus following S-nitrosylation on cysteine 152 that leads to its interaction with SIAH1, which contains a nuclear localization signal. In the nucleus it participates in transcription, RNA transport, DNA replication and apoptosis. Sulfhydration at cysteine 152 is reported to increase its catalytic activity. Its nuclear functions are attributed to its nitrosylase activity, which mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2, and DNA-PK. Oxidative stress is reported to promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process known as nucleocytoplasmic coagulation. These aggregates are observed in vivo in the affected tissues of patients with Alzheimer disease or alcoholic liver cirrhosis, or in cell cultures during necrosis. Oxidation at methionine 46 plays an essential role in the formation of these insoluble structures. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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