E6908
pFLAG-CMV™-5.1 Expression Vector
shuttle vector for intracellular transient expression of C-terminal FLAG
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€284.00
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1 G
€78.50
5 G
€284.00
About This Item
UNSPSC Code:
12352200
€78.50
Please contact Customer Service for Availability
Recommended Products
tag
FLAG® tagged
grade
for molecular biology
form
buffered aqueous solution
Peptide tag location
C-terminal
shipped in
dry ice
storage temp.
−20°C
1 of 4
This Item | M0206000 | 1375207 | SML2233 |
|---|---|---|---|
| assay >99% (HPLC) | assay - | assay - | assay ≥98% (HPLC) |
| Quality Level 200 | Quality Level - | Quality Level - | Quality Level - |
| solubility H2O: 50 mg/mL | solubility - | solubility - | solubility DMSO: 2 mg/mL, clear (warmed) |
| originator Novartis | originator - | originator - | originator - |
| color white | color - | color - | color white to beige |
General description
The pFLAG-CMV™-5.1 Expression Vector is a 4.7 kb derivative of the pCMV5 transient expression vector, used for establishing transient intracellular expression of C-terminal FLAG® fusion proteins in mammalian cells.
pFLAG-CMV-5.1 is a shuttle vector for E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using an SV40 T antigenexpressing host.
The pFLAG-CMV-5b-BAP Control Plasmid is a 6.0 kb derivative of the pCMV5 transient expression vector for intracellular expression of C-terminal FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.
Vector Maps and Sequences
pFLAG-CMV-5.1 is a shuttle vector for E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using an SV40 T antigenexpressing host.
The pFLAG-CMV-5b-BAP Control Plasmid is a 6.0 kb derivative of the pCMV5 transient expression vector for intracellular expression of C-terminal FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.
Vector Maps and Sequences
Application
pFLAG-CMV™-5.1 Expression Vector is suitable for transient or stable expression and secretion of N-terminal FLAG®-tagged fusion proteins in mammalian cells.
Components
- pFLAG-CMV™-5.1 Expression Vector 20 μg (E7901) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
- pFLAG-CMV™-5b-BAP Control Plasmid 20 μg (P5225) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
Principle
The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG®-fusion constructs. The multiple cloning region of the pFLAG-CMV™-5.1 vector is not preceded by a translational initiation signal. A translational start sequence must be included with the insert DNA. The multiple cloning region of the pFLAG-CMV-5.1 vector is compatible with other CMV vectors.
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
10 - Combustible liquids
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Monica Red Brewer et al.
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Proceedings of the National Academy of Sciences of the United States of America, 104(49), 19238-19243 (2007-11-29)
Structural studies of the extracellular and tyrosine kinase domains of the epidermal growth factor receptor (ErbB-1) provide considerable insight into facets of the receptor activation mechanism, but the contributions of other regions of ErbB-1 have not been ascertained. This study
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Human molecular genetics, 16(20), 2394-2410 (2007-07-04)
We characterized a family consisting of four mammalian proteins of unknown function (NKAIN1, 2, 3 and 4) and a single Drosophila ortholog dNKAIN. Aside from highly conserved transmembrane domains, NKAIN proteins contain no characterized functional domains. Striking amino acid conservation
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